Schlondorff D, Satriano J A, DeCandido S
Biochem Biophys Res Commun. 1986 Nov 26;141(1):39-45. doi: 10.1016/s0006-291x(86)80331-x.
Pertussis toxin may inactivate N proteins linked to phospholipase C. We examined the effect of pretreatment with pertussis toxin at different concentrations and times on agonist-induced PGE2 synthesis in mesangial cells. Two to four hours with 10-50 ng/ml of pertussis toxin inhibited the response to angiotensin and platelet activating factor, but with a different sensitivity. This was associated with decreased [14C]arachidonic acid release in prelabeled cells. The response to A23187 was unaltered. At high concentrations (1 to 5 micrograms/ml) pertussis toxin increased basal PGE2 and the response to all agonists. Pertussis toxin pretreatment resulted in a dose-dependent ribosylation of a 40 kDa protein band. Thus, responses to different agonists have different sensitivity to pertussis toxin inhibition, which at high concentrations may even have opposite effects.
百日咳毒素可能会使与磷脂酶C相关的N蛋白失活。我们研究了在不同浓度和时间用百日咳毒素预处理对系膜细胞中激动剂诱导的前列腺素E2（PGE2）合成的影响。用10 - 50纳克/毫升的百日咳毒素处理两到四个小时会抑制对血管紧张素和血小板活化因子的反应，但敏感性不同。这与预先标记的细胞中[14C]花生四烯酸释放减少有关。对A23187的反应未改变。在高浓度（1至5微克/毫升）时，百日咳毒素会增加基础PGE2以及对所有激动剂的反应。百日咳毒素预处理导致一条40千道尔顿蛋白带的剂量依赖性核糖基化。因此，对不同激动剂的反应对百日咳毒素抑制具有不同的敏感性，在高浓度时甚至可能产生相反的效果。
