Pfeilschifter J, Bauer C
Biochem J. 1986 May 15;236(1):289-94. doi: 10.1042/bj2360289.
Incubation of rat renal mesangial cells with angiotensin II (0.1 microM) resulted in transient breakdown of phosphatidylinositol 4,5-bisphosphate, rapid generation of diacylglycerol and phosphatidic acid, increased 45Ca2+ influx, increased intracellular [Ca2+] as measured by quin 2, and increased prostaglandin E2 synthesis. All of these processes were markedly inhibited time- and dose-dependently by prior exposure of cells to pertussis toxin. In contrast, the effects of the ionophore A23187 on 45Ca2+ influx and prostaglandin E2 synthesis were not altered by the exposure of the cells to pertussis toxin. The action of the toxin was not associated with alterations in cellular concentrations of cyclic AMP. Incubation of membrane fraction of mesangial cells with pertussis toxin resulted in ADP-ribosylation of Mr-42,000 protein. From all these results, it is likely that a G protein is involved in receptor-mediated signal transduction in renal mesangial cells.
用血管紧张素II（0.1微摩尔）孵育大鼠肾系膜细胞，导致磷脂酰肌醇4,5 - 二磷酸短暂分解，二酰基甘油和磷脂酸快速生成，45Ca2+内流增加，用喹啉2测量的细胞内[Ca2+]增加，以及前列腺素E2合成增加。所有这些过程在细胞预先暴露于百日咳毒素后，均受到时间和剂量依赖性的显著抑制。相反，离子载体A23187对45Ca2+内流和前列腺素E2合成的作用，在细胞暴露于百日咳毒素后未发生改变。毒素的作用与细胞内环磷酸腺苷浓度的改变无关。用百日咳毒素孵育系膜细胞膜组分，导致分子量为42,000的蛋白质发生ADP - 核糖基化。从所有这些结果来看，肾系膜细胞中可能有一个G蛋白参与受体介导的信号转导。
