Nishida Y, Hata M, Nishizuka Y, Rutter W J, Ebina Y
Biochem Biophys Res Commun. 1986 Dec 15;141(2):474-81. doi: 10.1016/s0006-291x(86)80197-8.
A Drosophila cDNA clone was obtained using the human insulin receptor cDNA sequence as a probe. The 3586 bp nucleotide sequence predicted a single polypeptide of 1095 amino acid residues which showed considerable homology (35.2%) with the human insulin receptor precursor. Although the cDNA was incomplete at its 5'-terminal region, it encodes a transmembrane glycoprotein as a single precursor of a two subunit molecule having a structural architecture similar to that of the human insulin receptor precursor. The presumptive beta subunit carries a well conserved Tyr kinase domain which showed 63.5% homology with that of human insulin receptor; however the protein of the alpha subunit is only weakly conserved (25%).
利用人胰岛素受体cDNA序列作为探针获得了一个果蝇cDNA克隆。3586 bp的核苷酸序列预测出一个由1095个氨基酸残基组成的单一多肽，它与人胰岛素受体前体具有相当高的同源性（35.2%）。尽管该cDNA在其5'-末端区域不完整，但它编码一种跨膜糖蛋白，作为具有与人胰岛素受体前体相似结构的双亚基分子的单一前体。推测的β亚基带有一个高度保守的酪氨酸激酶结构域，与人类胰岛素受体的酪氨酸激酶结构域具有63.5%的同源性；然而，α亚基的蛋白质仅具有较弱的保守性（25%）。
