Turk J Med Sci. 2019 Apr 18;49(2):696-702. doi: 10.3906/sag-1802-17.
BACKGROUND/AIM: The aim was to investigate the protective and therapeutic effects of ghrelin, which has antioxidant and antiinflammatory activity, on preventing kidney damage that occurs by induced partial ureteral obstruction in rats
Twenty-eight adult male rats were included in the study, and the rats were divided into 4 groups. After the laparotomy operation on the sham group, the ureter was identified in the retroperitoneal area and was duly sutured (n = 7). Ghrelin was administered for seven days intraperitoneally, and after the nephrectomy performed on the 15th day, the rats were sacrificed (n = 7). A partial ureteral obstruction was performed after the laparotomy on the PUO group. The rats were sacrificed after the nephrectomy operation performed on the 15th day (n = 7). A partial ureteral obstruction was formed after the laparotomy followed by seven days of waiting in the PUO + ghrelin group. Ghrelin was given in the dose of 10 ng/kg per day intraperitoneally for the next 7 days, and the rats were sacrificed after the nephrectomy operation performed on the 15th day (n = 7). All groups were evaluated for histological damage and catalase, superoxide dismutase, total glutathione, malondialdehyde, and myeloperoxidase levels were measured in the same tissues
When the 2nd group and the sham group were compared histologically, it was observed that the damage had increased by a statistically significant level in the partial ureteral obstruction group (P = 0.001). When the group which was ghrelin-treated after the partial ureteral obstruction was compared to the group with just partial ureteral obstruction, the histopathological changes were found to decrease significantly in that group (P = 0.001). While the statistical significance of the levels of CAT, GSH, and MPO enzymes was detected among biochemical changes in the 2nd group when compared to the sham group (P < 0.01), the 3rd group showed a statistically significant difference in the levels of SOD and GSH enzymes compared to the 4th group (P < 0.05).
Ghrelin administration to rats after the formation of an experimental partial unilateral ureteral obstruction reduces tissue damage due to ghrelin’s antiinflammatory and antioxidant effects. Ghrelin administration may prevent tissue damage biochemically and histopathologically in obstructive uropathy cases
背景/目的：本研究旨在探讨具有抗氧化和抗炎活性的 Ghrelin 对预防大鼠诱导性部分输尿管梗阻所致肾损伤的保护和治疗作用。
将 28 只成年雄性大鼠纳入研究，将大鼠分为 4 组。假手术组行剖腹手术后，在腹膜后区识别输尿管并适当缝合（n = 7）。Ghrelin 经腹腔内给药 7 天，第 15 天行肾切除术时处死大鼠（n = 7）。PUO 组行剖腹手术后行部分输尿管梗阻。第 15 天行肾切除术后处死大鼠（n = 7）。PUO + Ghrelin 组行剖腹手术后等待 7 天形成部分输尿管梗阻。Ghrelin 以 10ng/kg/天的剂量经腹腔内给药 7 天，第 15 天行肾切除术后处死大鼠（n = 7）。所有组均进行组织学损伤评估，并在相同组织中测量过氧化氢酶、超氧化物歧化酶、总谷胱甘肽、丙二醛和髓过氧化物酶水平。
与假手术组相比，第 2 组和第 2 组组织学观察到部分输尿管梗阻组损伤明显增加，差异具有统计学意义（P = 0.001）。与单纯部分输尿管梗阻组相比，部分输尿管梗阻后给予 Ghrelin 治疗的组，其组织病理学变化明显减少，差异具有统计学意义（P = 0.001）。与假手术组相比，第 2 组生化变化中 CAT、GSH 和 MPO 酶的水平具有统计学意义（P < 0.01），第 3 组与第 4 组相比 SOD 和 GSH 酶的水平具有统计学意义（P < 0.05）。
在形成实验性部分单侧输尿管梗阻后，Ghrelin 对大鼠的给药可减轻 Ghrelin 的抗炎和抗氧化作用引起的组织损伤。Ghrelin 给药可能会在生物化学和组织病理学上预防梗阻性尿路病例中的组织损伤。
