Department of Biochemistry, Faculty of Medicine, Bulent Ecevit University (Formerly Karaelmas University), Zonguldak, Turkey.
Kaohsiung J Med Sci. 2013 Feb;29(2):69-74. doi: 10.1016/j.kjms.2012.08.011. Epub 2012 Oct 18.
The purpose of this study was to investigate the effects of ghrelin on inflammatory response and tissue damage following trauma-induced acute lung injury. Thirty male wistar albino rats (300-400 g) were randomly assigned into three groups: control group (n = 6), lung contusion plus saline (saline-treated, n = 12), and lung contusion plus ghrelin (ghrelin-treated, n = 12). Saline- or ghrelin-treated traumatic rats were sacrificed at two time points (24 and 72 hours) after lung contusion. Blood was collected for the analysis of serum adenosine deaminase (ADA). Tissue transforming growth factor-beta 1 (TGF-β1) and matrix metalloproteinase-2 (MMP-2) levels were measured by enzyme-linked immunosorbent assay and histopathological examination was performed on the lung tissue samples. Our results indicated that ghrelin significantly reduced morphologic damages. Serum ADA activities were significantly decreased after lung contusion and this decline started early with ghrelin treatment. TGF-β1 and MMP-2 levels in lung tissue were elevated at 72 hours after lung contusion and treatment with ghrelin significantly increased TGF-β1 level and reduced MMP-2 level. In conclusion, our study demonstrates that acute lung injury initiated proinflammatory responses and ghrelin administration showed an anti-inflammatory effect in lung contusion.
本研究旨在探讨 ghrelin 对创伤性急性肺损伤后炎症反应和组织损伤的影响。30 只雄性 wistar 白化大鼠（300-400g）随机分为三组：对照组（n=6）、肺挫伤加生理盐水（生理盐水处理组，n=12）和肺挫伤加 ghrelin（ghrelin 处理组，n=12）。生理盐水或 ghrelin 处理的创伤性大鼠在肺挫伤后 24 和 72 小时两个时间点处死。采集血液用于分析血清腺苷脱氨酶（ADA）。通过酶联免疫吸附试验测定组织转化生长因子-β1（TGF-β1）和基质金属蛋白酶-2（MMP-2）水平，并对肺组织样本进行组织病理学检查。我们的结果表明，ghrelin 显著减轻了形态损伤。肺挫伤后血清 ADA 活性显著降低，ghrelin 治疗后这种下降开始较早。肺挫伤后 72 小时，肺组织中 TGF-β1 和 MMP-2 水平升高，ghrelin 治疗显著增加 TGF-β1 水平并降低 MMP-2 水平。总之，本研究表明，急性肺损伤引发了促炎反应，ghrelin 给药在肺挫伤中表现出抗炎作用。
