Voronov S E, Vertiev Iu V, Shaginian I A, Ezepchuk Iu V
Biull Eksp Biol Med. 1987 Jan;103(1):80-3.
The preparation and identification of B subunit of thermolabile enterotoxin produced by A-B+ gene-containing strain are described. The E. coli strain studied is shown to produce protein identical in its molecular properties and antigenic specificity to B subunit obtained from the whole thermolabile enterotoxin. Partial antigenic affinity between B subunits of thermolabile enterotoxin obtained from different sources and B subunit from cholera enterotoxin has been established in immunochemical studies. Electrophoretic and immunochemical analysis has confirmed the absence of A-subunit admixtures in B-subunit preparation obtained from /A-B+/E. coli strain.
本文描述了含A - B⁺基因菌株产生的不耐热肠毒素B亚单位的制备与鉴定。所研究的大肠杆菌菌株产生的蛋白质,其分子特性和抗原特异性与从完整不耐热肠毒素中获得的B亚单位相同。免疫化学研究已证实,从不同来源的不耐热肠毒素B亚单位与霍乱肠毒素B亚单位之间存在部分抗原亲和力。电泳和免疫化学分析证实,从/A - B⁺/大肠杆菌菌株获得的B亚单位制剂中不存在A亚单位混合物。