Yamamoto K, Takeda Y, Miwatani T, Craig J P
Infect Immun. 1983 Sep;41(3):896-901. doi: 10.1128/iai.41.3.896-901.1983.
Cholera-like enterotoxin produced by a non-O1 strain of Vibrio cholerae, S7 (S7 enterotoxin), isolated from human diarrheal stool, was purified, and its physicochemical, biological, and immunological properties were compared with those of cholera enterotoxin from V. cholerae O1 569B (CT) and an enterotoxin produced by another non-O1 V. cholerae (E8498 enterotoxin) reported previously (Yamamoto et al., Infect. Immun. 39:1128-1135, 1983). The purified S7 enterotoxin had physicochemical properties different from those of CT and E8498 enterotoxin. S7 enterotoxin had greater relative mobility in conventional polyacrylamide gel disc electrophoresis and a lower isoelectric point, and its B subunit was smaller than those of CT and E8498 enterotoxin. The results of sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis suggested that the size of the aggregate of the B subunits of S7 enterotoxin is larger than that of CT and E8498 enterotoxin. The biological and immunological properties of S7 enterotoxin were indistinguishable from those of CT and E8498 enterotoxin. These results indicate that non-O1 vibrios may produce more than one kind of cholera-like enterotoxin: one which is identical to CT (E8498 enterotoxin type) and another which is not identical to CT (S7 enterotoxin type).
从人类腹泻粪便中分离出的非O1群霍乱弧菌S7株产生的霍乱样肠毒素（S7肠毒素）被纯化，并将其物理化学、生物学和免疫学特性与霍乱弧菌O1 569B株的霍乱肠毒素（CT）以及先前报道的另一种非O1群霍乱弧菌产生的肠毒素（E8498肠毒素）进行了比较（Yamamoto等人，《感染与免疫》39:1128 - 1135，1983）。纯化后的S7肠毒素具有与CT和E8498肠毒素不同的物理化学特性。S7肠毒素在常规聚丙烯酰胺凝胶圆盘电泳中具有更大的相对迁移率和更低的等电点，其B亚基比CT和E8498肠毒素的B亚基小。十二烷基硫酸钠 - 聚丙烯酰胺平板凝胶电泳结果表明，S7肠毒素B亚基聚集体的大小大于CT和E8498肠毒素的。S7肠毒素的生物学和免疫学特性与CT和E8498肠毒素无法区分。这些结果表明，非O1群弧菌可能产生不止一种霍乱样肠毒素：一种与CT相同（E8498肠毒素类型），另一种与CT不同（S7肠毒素类型）。
