Du Mei, Liu Jingzhang, Wang Qiong, Wang Fengbang, Bi Lei, Ma Chunyan, Song Maoyong, Jiang Guibin
Key Laboratory of Environmental Nanotechnology and Health Effects, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Key Laboratory of Environmental Nanotechnology and Health Effects, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Science, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.
J Environ Sci (China). 2025 Jan;147:74-82. doi: 10.1016/j.jes.2023.10.017. Epub 2023 Oct 24.
Endocrine disruptors such as bisphenol A (BPA) adversely affect the environment and human health. Laccases are used for the efficient biodegradation of various persistent organic pollutants in an environmentally safe manner. However, the direct application of free laccases is generally hindered by short enzyme lifetimes, non-reusability, and the high cost of a single use. In this study, laccases were immobilized on a novel magnetic three-dimensional poly(ethylene glycol) diacrylate (PEGDA)-chitosan (CS) inverse opal hydrogel (LAC@MPEGDA@CS@IOH). The immobilized laccase showed significant improvement in the BPA degradation performance and superior storage stability compared with the free laccase. 91.1% of 100 mg/L BPA was removed by the LAC@MPEGDA@CS@IOH in 3 hr, whereas only 50.6% of BPA was removed by the same amount of the free laccase. Compared with the laccase, the outstanding BPA degradation efficiency of the LAC@MPEGDA@CS@IOH was maintained over a wider range of pH values and temperatures. Moreover, its relative activity of was maintained at 70.4% after 10 cycles, and the system performed well in actual water matrices. This efficient method for preparing immobilized laccases is simple and green, and it can be used to further develop ecofriendly biocatalysts to remove organic pollutants from wastewater.
双酚A（BPA）等内分泌干扰物会对环境和人类健康产生不利影响。漆酶可用于以环境安全的方式高效生物降解各种持久性有机污染物。然而，游离漆酶的直接应用通常受到酶寿命短、不可重复使用以及单次使用成本高的阻碍。在本研究中，漆酶被固定在一种新型磁性三维聚乙二醇二丙烯酸酯（PEGDA）-壳聚糖（CS）反蛋白石水凝胶（LAC@MPEGDA@CS@IOH）上。与游离漆酶相比，固定化漆酶在BPA降解性能上有显著提高，且具有优异的储存稳定性。LAC@MPEGDA@CS@IOH在3小时内去除了100 mg/L BPA中的91.1%，而相同量的游离漆酶仅去除了50.6%的BPA。与漆酶相比，LAC@MPEGDA@CS@IOH在更宽的pH值和温度范围内保持了出色的BPA降解效率。此外，其相对活性在10个循环后保持在70.4%，并且该系统在实际水体基质中表现良好。这种制备固定化漆酶的有效方法简单且环保，可用于进一步开发生态友好型生物催化剂以去除废水中的有机污染物。
