Bonate P L
Department of Pharmacology/Toxicology, Washington State University, Pullman 99163.
Anal Biochem. 1988 Nov 15;175(1):300-4. doi: 10.1016/0003-2697(88)90393-4.
A standard spectrophotometric method for the quantitation of urinary albumin using bromphenol blue is evaluated to determine whether this method could be used to quantify cerebrospinal fluid albumin. In the modified procedure, 200 microliters of sample is added to 3 ml of bromphenol blue solution and the absorbance is read spectrophotometrically at 610 nm. Using standards and controls, the results were compared with known values and found to be both precise and accurate. The bromphenol blue method was compared with an immunoturbidometric method and found to be more precise and accurate, easier to perform, and cost effective. When compared to other dye-binding methods the bromphenol blue method is unique in its extremely low linear range and limit of detection. A minor disadvantage was the increased sample size necessary to obtain the increased precision.
评估了一种使用溴酚蓝定量尿白蛋白的标准分光光度法，以确定该方法是否可用于定量脑脊液白蛋白。在改良程序中，将200微升样品加入到3毫升溴酚蓝溶液中，并在610纳米处用分光光度计读取吸光度。使用标准品和对照品，将结果与已知值进行比较，发现其既精确又准确。将溴酚蓝法与免疫比浊法进行比较，发现其更精确、准确，操作更简便且具有成本效益。与其他染料结合法相比，溴酚蓝法的线性范围和检测限极低，这一点很独特。一个小缺点是为了提高精密度需要增加样品量。
