Volk H D, Brocke S, Osawa H, Diamantstein T
Clin Exp Immunol. 1986 Oct;66(1):126-31.
Parental strain T lymphocyte injected into F1 mice respond to allogeneic MHC antigens and so induce the symptoms of a graft-versus-host reaction (GVHR). We have measured the local GVHR by the popliteal lymph node assay, and showed the suppression of the local GVHR in mice by treatment with the monoclonal antibody (MoAb) AMT-13 which is specific against the interleukin 2 (IL-2) receptor on activated mouse lymphocytes. The inhibitory effect of the AMT-13 administration was comparable with the suppression of the local GVHR by treatment with L3T4, an MoAb directed against the T helper subset. The L3T4 administration caused a dramatic decrease in the proportion of the cells with the L3T4 phenotype in the circulation and a marginal reduction of these cells in the lymph nodes. In contrast, the AMT-13 treated mice showed no changes in the distribution of the T lymphocyte subsets besides those in the GVHR-stimulated lymph nodes. Obviously, only the small subset of antigen-activated IL-2 receptor-bearing lymphocytes was influenced by treatment with AMT-13. MoAb directed against antigens whose expression is restricted to activated lymphocytes, such as the IL-2 receptor, might become useful for a short term immunosuppression with limited side effects.
将亲代品系T淋巴细胞注射到F1小鼠体内会对同种异体MHC抗原产生反应，从而引发移植物抗宿主反应（GVHR）的症状。我们通过腘淋巴结试验测定了局部GVHR，并显示用针对活化小鼠淋巴细胞上白细胞介素2（IL-2）受体的单克隆抗体（MoAb）AMT-13处理可抑制小鼠的局部GVHR。AMT-13给药的抑制作用与用针对T辅助亚群的MoAb L3T4处理对局部GVHR的抑制作用相当。给予L3T4会导致循环中具有L3T4表型的细胞比例急剧下降，并且淋巴结中这些细胞略有减少。相比之下，除了在GVHR刺激的淋巴结中，AMT-13处理的小鼠T淋巴细胞亚群的分布没有变化。显然，只有一小部分抗原活化的携带IL-2受体的淋巴细胞受到AMT-13处理的影响。针对其表达仅限于活化淋巴细胞的抗原（如IL-2受体）的单克隆抗体可能对短期免疫抑制有用且副作用有限。
