Jonker M, Goldstein G, Balner H
Transplantation. 1983 Jun;35(6):521-6. doi: 10.1097/00007890-198306000-00001.
Monoclonal antibodies specific for human T cell subsets have been tested for their immunosuppressive effect in a rhesus monkey skin graft model. Rhesus monkeys were injected i.v. daily with antibodies specific for helper T cells (OKT4 and 4A), for cytotoxic/suppressor T cells (OKT8A), or all peripheral T cells (OKT11A), and they received an allogeneic skin graft one or two days after the initial antibody treatment. The OKT4, 4A, and 11A antibodies prolonged skin graft survival, but OKT8A did not. All animals were carefully monitored regarding levels of T cell subsets and antibody formation to the injected monoclonal antibody. The relevant T cell subset was not eliminated from the circulation when OKT4 and OKT4A antibodies were given separately. The OKT4+ cells remained in the circulation coated with antibody. OKT4+ cells could no longer be demonstrated when both OKT4 and 4A were given simultaneously. However, this difference in effect on the OKT4+ cells did not influence skin graft survival time. All animals receiving monoclonal antibody treatment developed antimouse-Ig antibodies after 10 to 13 days of treatment, which presumably counteracted the effect of the antibodies. From these data it appears that the rhesus monkey is a useful animal model in which to investigate the potential of monoclonal antibodies against human lymphocyte subpopulations to modify and regulate the immune response in an orderly fashion.
已在恒河猴皮肤移植模型中测试了针对人类T细胞亚群的单克隆抗体的免疫抑制作用。给恒河猴每日静脉注射针对辅助性T细胞的抗体（OKT4和4A）、针对细胞毒性/抑制性T细胞的抗体（OKT8A）或所有外周T细胞的抗体（OKT11A），并在初次抗体治疗后的一天或两天给它们进行同种异体皮肤移植。OKT4、4A和11A抗体延长了皮肤移植的存活时间，但OKT8A抗体没有。仔细监测了所有动物的T细胞亚群水平以及针对注射的单克隆抗体的抗体形成情况。单独给予OKT4和OKT4A抗体时，相关的T细胞亚群并未从循环中消除。OKT4+细胞仍留在循环中并被抗体包被。同时给予OKT4和4A时，无法再检测到OKT4+细胞。然而，对OKT4+细胞的这种作用差异并未影响皮肤移植的存活时间。所有接受单克隆抗体治疗的动物在治疗10至13天后都产生了抗小鼠Ig抗体，这可能抵消了抗体的作用。从这些数据来看，恒河猴是一种有用的动物模型，可用于研究针对人类淋巴细胞亚群的单克隆抗体以有序方式改变和调节免疫反应的潜力。
