Fuduche Maxime, Davidson Sylvain, Boileau Céline, Wu Long-Fei, Combet-Blanc Yannick
Aix Marseille University, IRD, CNRS, Université de Toulon, Marseille, France.
Aix Marseille University, CNRS, LCB, Marseille, France.
Front Microbiol. 2019 Mar 19;10:534. doi: 10.3389/fmicb.2019.00534. eCollection 2019.
This work describes a novel, simple and cost-effective culture system, named the Micro-Oxygenated Culture Device (MOCD), designed to grow microorganisms under particularly challenging oxygenation conditions. Two microaerophilic magnetotactic bacteria, a freshwater strain MSR-1 and a marine sp. strain QH-2, were used as biological models to prove the efficiency of the MOCD and to evaluate its specifications. Using the MOCD, growth rates of MSR-1 and QH-2 increased by four and twofold, respectively, when compared to traditional growing techniques using simple bottles. Oxystat-bioreactors have been typically used and specifically designed to control low dissolved oxygen concentrations, however, the MOCD, which is far less sophisticated was proven to be as efficient for both MSR-1 and QH-2 cultures with regard to growth rate, and even better for MSR-1 when looking at cell yield (70% increase). The MOCD enables a wide range of oxygenation conditions to be studied, including different O-gradients. This makes it an innovative and ingenious culture device that opens up new parameters for growing microaerobic microorganisms.
这项工作描述了一种新颖、简单且经济高效的培养系统，名为微氧培养装置（MOCD），旨在在特别具有挑战性的氧合条件下培养微生物。两种微需氧趋磁细菌，一种淡水菌株MSR-1和一种海洋菌株QH-2，被用作生物学模型来证明MOCD的效率并评估其规格。与使用简单瓶子的传统培养技术相比，使用MOCD时，MSR-1和QH-2的生长速率分别提高了四倍和两倍。氧气稳定生物反应器通常被使用并专门设计用于控制低溶解氧浓度，然而，事实证明，远没有那么复杂的MOCD在生长速率方面对MSR-1和QH-2培养同样有效，而从细胞产量来看（增加了70%），对MSR-1甚至更好。MOCD能够研究广泛的氧合条件，包括不同的氧气梯度。这使其成为一种创新且巧妙的培养装置，为培养微需氧微生物开辟了新的参数。
