Department of Orthodontics, São Leopoldo Mandic, R. Dr. José Rocha Junqueira, Campina, São Paulo, Brazil.
J Biophotonics. 2019 Oct;12(10):e201800311. doi: 10.1002/jbio.201800311. Epub 2019 Jul 8.
This study investigated the effects of photobiomodulation (PBM) on upper molar intrusion movement, regarding acceleration of orthodontic movement and its molecular effects. The sample consisted of 30 patients with indication of tooth intrusion for oral rehabilitation. Teeth were divided into three different groups: G1 (n = 10) pre-molars without force or laser application (control); G2 (n = 10) upper molar intrusion; and G3 (n = 10) upper molar intrusion and PBM. On PBM treated molars, the teeth were irradiated with a low-power diode laser (808 nm, 100 mW), receiving 1 J per point, density of 25 J/cm , with application of 10 s per point, 10 points (5 per vestibular and 5 per palatal region). Orthodontic force of intrusion applied every 30 days and PBM was performed immediately, 3 and 7 days after force application for 3 months. Gingival crevicular fluid was collected at the same time periods as the laser applications and interleukins (IL) 1-β, -6 and -8 were evaluated by enzyme-linked immunosorbent assay. Clinical measures were performed monthly to verify the amount of intrusion. The levels of IL-6, IL-8 and IL-1β increased under orthodontic force (G2 and G3) when compared to control group (G1), however, the cytokines levels were significantly higher after PBM (G3). The mean intrusion velocity was 0.26 mm/month in the irradiated group (G3), average duration of 8 months vs 0.17 mm/month for the non-irradiated group (G2), average duration of 12 months. This study suggests that PBM accelerates tooth movement during molar intrusion, due to modulation of IL-6, IL-8 and IL-1β during bone remodeling.
本研究旨在探讨光生物调节（PBM）对上颌磨牙内收移动的影响，包括加速正畸移动及其分子效应。该研究纳入了 30 名因口腔修复需要进行牙齿内收的患者。将牙齿分为三组：G1 组（n=10），无磨牙内收力或激光应用（对照组）；G2 组（n=10），上颌磨牙内收；G3 组（n=10），上颌磨牙内收和 PBM。在 PBM 治疗的磨牙上，使用低功率二极管激光（808nm，100mW）照射牙齿，每个点照射 1J，密度为 25J/cm2，每个点照射 10s，10 个点（5 个颊侧，5 个腭侧）。每隔 30 天施加一次正畸内收力，在施加力后第 3、7 天立即进行 PBM，共进行 3 个月。同时在激光应用的同期采集龈沟液，通过酶联免疫吸附试验评估白细胞介素（IL）-1β、-6 和 -8 的水平。每月进行临床测量以验证内收量。与对照组（G1）相比，在 G2 和 G3 组中，施加正畸力后，IL-6、IL-8 和 IL-1β 的水平增加，但在 PBM 后（G3），细胞因子水平显著升高。照射组（G3）的平均内收速度为 0.26mm/月，照射时间为 8 个月；未照射组（G2）的平均内收速度为 0.17mm/月，照射时间为 12 个月。本研究表明，PBM 通过调节骨重建过程中的 IL-6、IL-8 和 IL-1β，加速了磨牙内收过程中的牙齿移动。
