State Key Laboratory of Animal Nutrition, Department of Animal Nutrition and Feed Science , China Agricultural University , Beijing 100193 , China.
Department of Animal Science , Texas A&M University , College Station , Texas 77843 , United States.
J Agric Food Chem. 2019 May 1;67(17):4915-4922. doi: 10.1021/acs.jafc.9b00942. Epub 2019 Apr 19.
Lung injury is a complicated and lethal condition characterized by alveolar barrier disruption, pulmonary edema, enhanced inflammation, and apoptosis in alveoli. However, therapeutic strategies to ameliorate lung injury without exerting side effects are not available. Functional amino acids have been shown to have anti-inflammatory and anti-apoptotic effects under various conditions. The objective of this study was to test the hypothesis that arginine, glutamine, or glycine supplementation ameliorated lipopolysaccharide (LPS)-induced lung injury in mice. Mice pretreated with aerosolized arginine, glutamine, or glycine were exposed to aerosolized LPS to induce lung injury. Results showed that arginine or glycine pretreatment beneficially reduced LPS-induced collagen deposition, apoptosis of alveolar cells, expression of inflammatory cytokines and chemokines, and accumulation of neutrophils and macrophages in lung tissues of mice, thus contributing to improved alveolar integrity and function. Glutamine administration reduced LPS-induced collagen deposition and inflammatory cytokines without affecting any other parameters examined in the study. Our findings indicated that arginine or glycine pretreatment effectively alleviated LPS-induced lung injury by inhibiting the accumulation of lymphocytes, the release of inflammatory cytokines and chemokines, and the apoptosis of alveolar cells. Supplementation of arginine or glycine may be a novel nutritional strategy to reduce deleterious effects of bacterial infection on alveolar function.
肺损伤是一种复杂且致命的病症，其特征为肺泡屏障破坏、肺水肿、炎症增强和肺泡细胞凋亡。然而，目前尚无改善肺损伤而无副作用的治疗策略。功能性氨基酸在各种情况下均显示出抗炎和抗凋亡作用。本研究旨在验证以下假设，即精氨酸、谷氨酰胺或甘氨酸补充可改善脂多糖（LPS）诱导的小鼠肺损伤。用雾化的精氨酸、谷氨酰胺或甘氨酸预处理小鼠，然后用雾化的 LPS 诱导肺损伤。结果表明，精氨酸或甘氨酸预处理可有益地减少 LPS 诱导的胶原蛋白沉积、肺泡细胞凋亡、炎症细胞因子和趋化因子的表达以及中性粒细胞和巨噬细胞在肺组织中的积聚，从而有助于改善肺泡完整性和功能。谷氨酰胺给药可减少 LPS 诱导的胶原蛋白沉积和炎症细胞因子，而不影响研究中检查的任何其他参数。我们的研究结果表明，精氨酸或甘氨酸预处理通过抑制淋巴细胞的积聚、炎症细胞因子和趋化因子的释放以及肺泡细胞的凋亡，有效地缓解了 LPS 诱导的肺损伤。补充精氨酸或甘氨酸可能是减少细菌感染对肺泡功能的有害影响的一种新的营养策略。
