Department of Orthopedic, Daqing Oilfield General Hospital, Daqing, China.
Eur Rev Med Pharmacol Sci. 2019 Apr;23(7):2710-2718. doi: 10.26355/eurrev_201904_17541.
The aim of this study was to investigate the expression level of micro-ribonucleic acid-1207-5p (miR-1207-5p) in steroid-induced necrosis of femoral head (SNFH) and its correlation with SNFH. Meanwhile, we also aimed to analyze the relationship between miR-1207-5p expression and vascular endothelial growth factor (VEGF) in the femoral head.
From May 2016 to December 2017, 60 patients aged (55.4±8.7) were selected in our hospital. All patients were diagnosed and confirmed as SNFH. Total RNA was extracted from the necrotic femoral head tissues and peripheral blood. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was used to detect the expression level of miR-1207-5p in tissues. At the same time, immunohistochemistry and Western blotting were adopted to detect VEGF expression in the bone tissue of patients with high or low expression of miR-1207-5p. 7 patients with femoral neck fracture aged (45.6±4.51) were enrolled in the control group. In the animal experiment, the rat SNFH model was established by intraperitoneal injection of lipopolysaccharide and methylprednisolone. Subsequently, the expression levels of miR-1207-5p and VEGF in necrotic femoral tissues were detected. Meanwhile, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was applied to detect cell apoptosis in bone lacunae of miR-1207-5p high expression group and miR-1207-5p low expression group, respectively.
The expression level of miR-1207-5p in the necrotic bone tissue of the SNFH group was significantly higher than that of the control group. The expression level of miR-1207-5p was inversely proportional to Harris Hip score (p<0.05). A higher expression of miR-1207-5p indicated a lower expression level of VEGF (p<0.05). The animal experimental results revealed that miR-1207-5p expression in the necrotic femoral head tissue of SNFH group was significantly higher than that of the control group. Furthermore, the number of apoptotic cells in bone lacunae was remarkably higher in miR-1207-5p high expression group (p<0.05).
MiR-1207-5p is significantly up-regulated in necrotic femoral head tissue and serum of SNFH patients. Meanwhile, its expression level is inversely proportional to Harris Hip score of patients. The possible underlying mechanism may be related to the inhibitory effect of miR-1207-5p on VEGF.
本研究旨在探讨微小 RNA-1207-5p（miR-1207-5p）在激素诱导性股骨头坏死（SNFH）中的表达水平及其与 SNFH 的相关性。同时，我们还旨在分析 miR-1207-5p 表达与股骨头血管内皮生长因子（VEGF）之间的关系。
2016 年 5 月至 2017 年 12 月，我院收治 60 例年龄（55.4±8.7）岁的患者。所有患者均经诊断和证实为 SNFH。从坏死股骨头组织和外周血中提取总 RNA。采用逆转录-聚合酶链反应（RT-PCR）检测组织中 miR-1207-5p 的表达水平。同时，采用免疫组化和 Western blot 检测 miR-1207-5p 高表达和低表达患者骨组织中 VEGF 的表达。纳入对照组 7 例年龄（45.6±4.51）岁的股骨颈骨折患者。在动物实验中，通过腹腔注射脂多糖和甲基强的松龙建立大鼠 SNFH 模型。随后，检测坏死股骨头组织中 miR-1207-5p 和 VEGF 的表达水平。同时，采用末端脱氧核苷酸转移酶 dUTP 缺口末端标记（TUNEL）染色分别检测 miR-1207-5p 高表达组和 miR-1207-5p 低表达组骨陷窝细胞的凋亡情况。
SNFH 组坏死骨组织中 miR-1207-5p 的表达水平明显高于对照组。miR-1207-5p 的表达水平与 Harris 髋关节评分呈负相关（p<0.05）。miR-1207-5p 表达水平越高，VEGF 表达水平越低（p<0.05）。动物实验结果显示，SNFH 组坏死股骨头组织中 miR-1207-5p 的表达明显高于对照组。此外，miR-1207-5p 高表达组骨陷窝细胞凋亡数量明显增多（p<0.05）。
miR-1207-5p 在 SNFH 患者坏死股骨头组织和血清中显著上调，其表达水平与患者的 Harris 髋关节评分呈负相关。其潜在机制可能与 miR-1207-5p 对 VEGF 的抑制作用有关。
