Li Liangyuan, Lu Shouliang, Ma Qiman, Wan Pengcheng, Liu Changbin, Yang Hanyulu, Yang Hua, Yang Yonglin, Dai Rong, Shi Guoqing
College of Animal Science and Technology, Shihezi University, Shihezi, China.
State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, China.
Reprod Domest Anim. 2019 Jun;54(6):892-901. doi: 10.1111/rda.13441. Epub 2019 May 10.
The aim of this research was to investigate the changes in reproductive hormone receptor expressions of the ovary and hormone concentrations between oestrous cycle pattern of two different sheep breeds in China. Ovarian tissues were collected from Chinese Merino (Junken type) and Hu sheep with different reproductive states in spring and autumn. Serum samples were assayed for oestrogen (E2), progesterone (P), luteinizing hormone (LH) and follicle-stimulating hormone (FSH) concentrations by radioimmunoassay during spring. The ovarian expression of hormone receptors (ERα, ERβ, PR, LHR and FSHR) was analysed using real-time reverse transcription polymerase chain reaction (RT-PCR). In Chinese Merino, there was no corpora lutea and ovulation point on the surfaces of ovaries in spring and low basal levels of both LH and P in serum. ERα, ERβ and FSHR were expressed significantly higher in Merino ovaries during anoestrus compared with oestrous or luteal phases of Hu sheep (p < 0.05 or p < 0.01). However, both varieties of sheep exhibited a similar tendency to secrete E2 and FSH. Compared with Hu sheep, FSH levels were slightly higher in Merino serum. In Hu sheep, ERα, ERβ, FSHR, LHR and PR expressed in luteal phase ovaries during spring were significantly lower (p < 0.05, p < 0.01 or p < 0.001) than autumn. Interestingly, LHR and PR expressed in anoestrous ovaries were similar to that in oestrous phase of both sheep breeds. The above results suggest that seasonal reproductive sheep increased the expression of E2 and FSH receptors in ovary during spring may enhance the effects of E2 and FSH on follicular development. It is likely that this enhancement prevents the ovary from progressing to the luteal phase.
本研究旨在探讨中国两种不同绵羊品种发情周期模式下卵巢生殖激素受体表达及激素浓度的变化。于春秋两季采集处于不同生殖状态的中国美利奴（军垦型）绵羊和湖羊的卵巢组织。在春季，采用放射免疫分析法检测血清样本中雌激素（E2）、孕酮（P）、促黄体生成素（LH）和促卵泡生成素（FSH）的浓度。运用实时逆转录聚合酶链反应（RT-PCR）分析卵巢中激素受体（ERα、ERβ、PR、LHR和FSHR）的表达情况。在中国美利奴绵羊中，春季卵巢表面无黄体和排卵点，血清中LH和P的基础水平较低。与湖羊发情期或黄体期相比，美利奴绵羊卵巢在乏情期时ERα、ERβ和FSHR的表达显著更高（p < 0.05或p < 0.01）。然而，两个品种的绵羊分泌E2和FSH的趋势相似。与湖羊相比，美利奴绵羊血清中的FSH水平略高。在湖羊中，春季黄体期卵巢中ERα、ERβ、FSHR、LHR和PR的表达显著低于秋季（p < 0.05、p < 0.01或p < 0.001）。有趣的是，两个品种绵羊乏情期卵巢中LHR和PR的表达与发情期相似。上述结果表明，季节性繁殖的绵羊在春季增加卵巢中E2和FSH受体的表达可能会增强E2和FSH对卵泡发育的作用。这种增强作用可能会阻止卵巢进入黄体期。
