He Xiaoyun, Tao Lin, Zhong Yingjie, Di Ran, Xia Qing, Wang Xiangyu, Guo Xiaofei, Gan Shangquan, Zhang Xiaosheng, Zhang Jinlong, Liu Qiuyue, Chu Mingxing
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Tianjin Institute of Animal Sciences, Tianjin, China.
PeerJ. 2021 Apr 21;9:e10953. doi: 10.7717/peerj.10953. eCollection 2021.
The pituitary is a vital endocrine organ that regulates animal seasonal reproduction by controlling the synthesis and secretion of the hormone. The change of photoperiod is the key factor affecting the function of the pituitary in animals, but the mechanism is unclear. Here, we studied the transcriptomic variation in pars distalis (PD) of the pituitary between short photoperiod (SP) and long photoperiod (LP) using RNA sequencing based on the OVX+E sheep. 346 differentially expressed (DE) lncRNAs and 186 DE-mRNA were found in the PD. Moreover, function annotation analysis indicated that the reproductive hormones and photoperiod response-related pathways including aldosterone synthesis and secretion, insulin secretion, thyroid hormone synthesis, and circadian entrainment were enriched. The interaction analysis of mRNA-lncRNA suggested that MSTRG.240648, MSTRG.85500, MSTRG.32448, and MSTRG.304959 targeted and , which may be involved in the photoperiodic regulation of the PD. These findings provide resources for further study on the seasonal reproductive in ewes.
垂体是一个重要的内分泌器官，通过控制激素的合成和分泌来调节动物的季节性繁殖。光周期的变化是影响动物垂体功能的关键因素，但其机制尚不清楚。在此，我们基于去势+雌激素处理的绵羊，利用RNA测序研究了短光周期（SP）和长光周期（LP）下垂体远侧部（PD）的转录组变异。在PD中发现了346个差异表达的lncRNA和186个差异表达的mRNA。此外，功能注释分析表明，包括醛固酮合成与分泌、胰岛素分泌、甲状腺激素合成和昼夜节律调节在内的生殖激素和光周期反应相关途径得到了富集。mRNA-lncRNA的相互作用分析表明，MSTRG.240648、MSTRG.85500、MSTRG.32448和MSTRG.304959靶向[具体基因未给出]，这可能参与了PD的光周期调节。这些发现为进一步研究母羊季节性繁殖提供了资源。
