Department of Physiology & Pharmacology, The University of Western Ontario, London, Ontario, Canada.
Biotron Research Centre, The University of Western Ontario, London, Ontario, Canada.
PLoS One. 2019 Apr 19;14(4):e0215611. doi: 10.1371/journal.pone.0215611. eCollection 2019.
Limited information is available on how fetal growth retardation (FGR) affects the lung in the neonatal period in males and females. This led us to test the hypothesis that FGR alters lung mechanics and the surfactant system during the neonatal period. To test this hypothesis a model of FGR was utilized in which pregnant rat dams were fed a low protein diet during both the gestation and lactation period. We subsequently analyzed lung mechanics using a FlexiVent ventilator in male and female pups at postnatal day 7 and 21. Lung lavage material was obtained at postnatal day 1, 7 and 21, and was used for analysis of the surfactant system which included measurement of the pool size of surfactant and its subfraction as well as the surface tension reducing ability of the surfactant. The main result of the study was a significantly lower lung compliance and higher tissue elastance which was observed in FGR female offspring at day 21 compared to control offspring. In addition, female LP offspring exhibited lower surfactant pool sizes at postnatal day 1compared to controls. These changes were not observed in the male offspring. It is concluded that FGR has a different impact on pulmonary function and on surfactant in female, as compared to male, offspring.
关于胎儿生长迟缓（FGR）如何影响男性和女性新生儿期肺部的信息有限。这促使我们检验这样一个假设，即 FGR 在新生儿期会改变肺力学和表面活性剂系统。为了验证这一假设，我们在妊娠和哺乳期都用低蛋白饮食喂养孕鼠，建立了 FGR 模型。随后，我们在出生后第 7 天和第 21 天利用 FlexiVent 通气机对雄性和雌性幼崽的肺力学进行了分析。在出生后第 1、7 和 21 天收集肺灌洗液材料，用于分析表面活性剂系统，包括表面活性剂池大小及其亚组分以及表面活性剂降低表面张力的能力。研究的主要结果是，与对照组相比，FGR 雌性后代在第 21 天的肺顺应性明显降低，组织弹性增加。此外，LP 雌性后代在出生后第 1 天的表面活性剂池大小低于对照组。在雄性后代中未观察到这些变化。结论是，与雄性后代相比，FGR 对女性后代的肺功能和表面活性剂有不同的影响。
