Department of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro, Tokyo, 153-8902, Japan; Determent of Creative Research, Exploratory Research Center on Life and Living Systems (ExCELLS), Myodaiji, Okazaki, Aichi, 444-8585, Japan.
Department of Chemistry, Faculty of Science, Kanagawa University, Tsuchiya, Hiratsuka, Kanagawa, 259-1293, Japan.
Chem Phys Lipids. 2019 Aug;222:1-7. doi: 10.1016/j.chemphyslip.2019.04.007. Epub 2019 Apr 16.
A novel phosphoric membrane lipid (phospholipid) bearing an oleyl group as one of the hydrophobic chains formed a liposome with a thin lamella in water. Since the anionic membrane of pre-existing liposomes acted as a catalytic surface in phosphate buffer, membrane lipids could be generated from their precursor in an autocatalytic manner without the inclusion of catalytic amphiphiles in the liposome. The morphological changes of this anionic liposome were monitored both by flow cytometry and optical microscopy, and it was found that the liposomes deformed into a budding shape, followed by division, after the addition of a membrane precursor. Hence, this anionic monocomponent liposome could be regarded as a sustainable self-reproducing system. This liposome was also able to provide a reaction cavity for enzymatic reactions, such as DNA amplification by a polymerase chain reaction.
一种新型的磷酸膜脂质（磷脂），其疏水性链之一为油基，在水中形成具有薄片状层的脂质体。由于预先存在的脂质体的阴离子膜在磷酸盐缓冲液中充当催化表面，因此可以在没有包含在脂质体中的催化两亲分子的情况下，以自催化的方式从其前体生成膜脂质。通过流式细胞术和光学显微镜监测这种阴离子脂质体的形态变化，发现添加膜前体后，脂质体变形为出芽形状，然后再分裂。因此，这种阴离子单一组分脂质体可以被视为一种可持续的自我繁殖系统。这种脂质体还可以为酶反应提供反应腔，例如聚合酶链式反应（PCR）的 DNA 扩增。
