IGEPP, INRA, Agrocampus-Ouest, Université de Rennes 1, 35650, Le Rheu, France; Centre Mondial de l'Innovation-Laboratoire de Nutrition Végétale Pôle Biocontrôle, Groupe Roullier, Saint Malo, France.
IGEPP, INRA, Agrocampus-Ouest, Université de Rennes 1, 35650, Le Rheu, France.
Infect Genet Evol. 2019 Sep;73:81-92. doi: 10.1016/j.meegid.2019.04.011. Epub 2019 Apr 17.
The cyst nematode Heterodera carotae, which parasitizes carrot roots, has been recorded in many countries in Europe (Italy, The Netherlands, Switzerland, France, Denmark, …), in South Africa and in North America (Canada, USA). To date, there is a lack of knowledge about the genetic structure of the populations of this economically important nematode. The aim of this work was to study the structuration of the genetic diversity of the carrot cyst nematode at the European scale. We have developed a set of thirteen polymorphic microsatellite markers and used it to genotype seventeen European populations of H. carotae coming from France, Switzerland, Italy, Denmark and one non-European population from Canada. As previously showed for other cyst nematode species, the H. carotae populations were characterised by a strong heterozygote deficit. A Bayesian clustering analysis revealed two distinct genetic clusters, with one group located in the north of Europe and a second one located in the south of Europe. Moreover, our results highlighted rather limited gene flow at small spatial scale and some events of long distance migration. This first investigation of the genetic diversity of H. carotae populations would be useful to develop sustainable control strategies.
胡萝卜胞囊线虫 Heterodera carotae 寄生在胡萝卜的根部，已在欧洲的许多国家（意大利、荷兰、瑞士、法国、丹麦等）、南非和北美（加拿大、美国）记录到。迄今为止，关于这种经济上重要的线虫种群的遗传结构知之甚少。这项工作的目的是研究胡萝卜胞囊线虫在欧洲范围内的遗传多样性结构。我们开发了一套 13 个多态性微卫星标记，并用于对来自法国、瑞士、意大利、丹麦的 17 个欧洲胡萝卜胞囊线虫种群和一个来自加拿大的非欧洲种群进行基因分型。与以前对其他胞囊线虫物种的研究一样，H. carotae 种群的特征是强烈的杂合子缺失。贝叶斯聚类分析显示存在两个不同的遗传群，一个群位于欧洲北部，另一个群位于欧洲南部。此外，我们的研究结果还表明，在小的空间尺度上基因流相当有限，并且存在一些长距离迁移事件。这是对 H. carotae 种群遗传多样性的首次调查，将有助于制定可持续的控制策略。
