用于通过“竞争性排除”处理雏鸡以预防沙门氏菌定植的推荐检测方法。
Recommended Assay for Treatment of Chicks to Prevent Salmonella Colonization by 'Competitive Exclusion'.
作者信息
Mead Geoffrey C, Barrow Paul A, Hinton Michael H, Humbert Florence, Impey Clive S, Lahellec Cecile, Mulder Roel W A W, Stavric Stanka, Stern Norman J
机构信息
Agricultural and Food Research Council Institute of Food Research, Bristol Laboratory, Langford, Bristol BS18 7DY, UK.
Agricultural and Food Research Council Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon PE17 2DA, Cambs., UK.
出版信息
J Food Prot. 1989 Jul;52(7):500-502. doi: 10.4315/0362-028X-52.7.500.
An assay is described for evaluating live-culture treatment material that may be given orally to chicks to prevent intestinal colonization by non-host-specific salmonellae. Both pre-treated and control chicks are challenged with 10 salmonellae/chick, using a strain bearing an antibiotic resistance marker. Chicks are examined 5 d after challenge to determine both the proportion of positive birds in treated and control groups and the levels of Salmonella in the caeca of infected individuals. The efficacy of the treatment is determined by calculation of values for Infection Factor and Protection Factor.
本文描述了一种用于评估活菌培养治疗材料的分析方法，该材料可口服给雏鸡，以防止非宿主特异性沙门氏菌在肠道定植。使用携带抗生素抗性标记的菌株，对预处理雏鸡和对照雏鸡均以每只雏鸡10个沙门氏菌进行攻毒。攻毒后5天检查雏鸡，以确定治疗组和对照组中阳性鸡的比例以及感染个体盲肠中的沙门氏菌水平。通过计算感染因子和保护因子的值来确定治疗效果。
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