胰岛内分泌聚集体的同种异体移植。

Allotransplantation of islet endocrine aggregates.

作者信息

Kakizaki K, Basadonna G, Merrell R C

出版信息

Diabetes. 1987 Mar;36(3):315-9. doi: 10.2337/diab.36.3.315.

DOI:10.2337/diab.36.3.315

Abstract

Dissociated pancreatic islets form endocrine aggregates from single-cell suspension by rotation culture. Islet cell aggregates, or neoislets, can provide endocrine reconstitution for diabetic rats and enjoy prolonged graft survival when neoislets are transplanted across a major histocompatibility barrier (Lewis to ACI). Long-term survival of grafted neoislets was obtained in 71% of recipients without any immunosuppression and in 100% of recipients with minimal immunosuppression. As predicted by cell-cell recognition in rotation-mediated aggregation, neoislets apparently exclude mesenchymal cells that bear la antigens. Therefore, reduced immunogenicity is accomplished.

摘要

通过旋转培养，解离的胰岛可从单细胞悬液形成内分泌聚集体。胰岛细胞聚集体，即新胰岛，可为糖尿病大鼠提供内分泌重建，并且当新胰岛跨越主要组织相容性屏障（从刘易斯大鼠到ACI大鼠）进行移植时，其移植存活时间会延长。在71%未接受任何免疫抑制的受体以及100%接受最小免疫抑制的受体中，移植的新胰岛实现了长期存活。正如旋转介导的聚集中细胞间识别所预测的那样，新胰岛显然排斥带有Ia抗原的间充质细胞。因此，实现了免疫原性的降低。