Kakizaki K, Matsuno S, Miyagawa K, Nakamura R
First Department of Surgery, Tohoku University, School of Medicine, Sendai, Japan.
Nihon Geka Gakkai Zasshi. 1988 Feb;89(2):245-50.
Dissociated pancreatic islets form aggregates from single cell suspension by rotation culture. Islet cell aggregates, or neoislets, newly formed from Lewis islet cells were transplanted into diabetic ACI rats intraportally and prolongation of neoislet grafts was observed (mean survival time greater than 45.8 +/- 6.4 days). Five out of seven recipients were still normoglycemic at 60 days after transplantation. Transplantation of neoislets combined with 3 day cyclosporine therapy for recipients resulted in 100% survival at 60 days after transplantation. These findings indicate that neoislets can provide endocrine constitution for streptozotocin diabetic rats and enjoy prolonged survival. As predicted by cell-cell recognition in rotation mediated aggregation, neoislets can exclude mesenchymal cells which bear Ia antigen. Therefore reduced immunogenicity is accomplished.
解离的胰岛通过旋转培养从单细胞悬液形成聚集体。将由Lewis胰岛细胞新形成的胰岛细胞聚集体或新胰岛经门静脉移植到糖尿病ACI大鼠体内，观察到新胰岛移植物的存活期延长（平均存活时间大于45.8±6.4天）。7只受体中有5只在移植后60天仍维持正常血糖水平。新胰岛移植并结合对受体进行3天的环孢素治疗，移植后60天的存活率达100%。这些结果表明，新胰岛可为链脲佐菌素诱导的糖尿病大鼠提供内分泌功能，且存活期延长。正如在旋转介导的聚集中细胞间识别所预测的那样，新胰岛可排除带有Ia抗原的间充质细胞。因此实现了免疫原性降低。
