Department of Medicine, School of Medicine, University of California, San Diego, 9500 Gilman Drive, San Diego, CA, 92093, USA.
Department of Medicine, Autonomous University of Barcelona, Plaça Cívica, Bellaterra, 08193, Barcelona, Spain.
Metabolomics. 2019 Apr 19;15(4):65. doi: 10.1007/s11306-019-1527-0.
Eicosanoids are biological lipids that serve as both activators and suppressors of inflammation. Eicosanoid pathways are implicated in synovitis and joint destruction in inflammatory arthritis, yet they might also have a protective function, underscoring the need for a comprehensive understanding of how eicosanoid pathways might be imbalanced. Until recently, sensitive and scalable methods for detecting and quantifying a high number of eicosanoids have not been available.
Here, we intend to describe a detailed eicosanoid profiling in patients with psoriatic arthritis (PsA) and evaluate correlations with parameters of disease activity.
Forty-one patients with PsA, all of whom satisfied the CASPAR classification criteria for PsA, were studied. Outcomes reflecting the activity of peripheral arthritis as well as skin psoriasis, Disease Activity Score (DAS)28, Clinical Disease Index (CDAI) and Body Surface Area (BSA) were assessed. Serum eicosanoids were determined by LC-MS, and the correlation between metabolite levels and disease scores was evaluated.
Sixty-six eicosanoids were identified by reverse-phase LC/MS. Certain eicosanoids species including several pro-inflammatory eicosanoids such as PGE2, HXB3 or 6,15-dk,dh,PGF1a correlated with joint disease score. Several eicosapentaenoic acid (EPA)-derived eicosanoids, which associate with anti-inflammatory properties, such as 11-HEPE, 12-HEPE and 15-HEPE, correlated with DAS28 (Disease Activity Score) and CDAI (Clinical Disease Activity Index) as well. Of interest, resolvin D1, a DHA-derived anti-inflammatory eicosanoid, was down-regulated in patients with high disease activity.
Both pro- and anti-inflammatory eicosanoids were associated with joint disease score, potentially representing pathways of harm as well as benefit. Further studies are needed to determine whether these eicosanoid species might also play a role in the pathogenesis of joint inflammation in PsA.
二十烷类是生物脂质，既是炎症的激活剂又是抑制剂。二十烷类途径与炎症性关节炎中的滑膜炎和关节破坏有关，但它们也可能具有保护作用，这突显了全面了解二十烷类途径如何失衡的必要性。直到最近，还没有用于检测和定量大量二十烷类的敏感和可扩展方法。
本研究旨在描述银屑病关节炎（PsA）患者的详细二十烷类谱，并评估其与疾病活动参数的相关性。
研究了 41 名符合 CASPAR 分类标准的银屑病关节炎患者。评估了反映外周关节炎和皮肤银屑病活动的结果，包括疾病活动评分（DAS）28、临床疾病指数（CDAI）和体表面积（BSA）。通过 LC-MS 测定血清二十烷类，评估代谢物水平与疾病评分的相关性。
通过反相 LC/MS 鉴定了 66 种二十烷类。某些二十烷类物质，包括几种促炎二十烷类物质，如 PGE2、HXB3 或 6,15-dk，dh，PGF1a，与关节疾病评分相关。几种来源于二十碳五烯酸（EPA）的二十烷类物质，如 11-HEPE、12-HEPE 和 15-HEPE，与 DAS28（疾病活动评分）和 CDAI（临床疾病活动指数）相关。有趣的是，具有抗炎作用的 DHA 衍生的二十烷类物质 17-Resolvin D1 在疾病活动度高的患者中下调。
促炎和抗炎二十烷类物质均与关节疾病评分相关，这可能代表了损害和获益的途径。需要进一步的研究来确定这些二十烷类物质是否也可能在 PsA 关节炎症的发病机制中发挥作用。
