Department of Orthodontics, Dental Hospital, Shanxi Medical University, Taiyuan, China.
Suzhou Dental Doctor Outpatient Department Co. LTD, Suzhou, China.
Mol Cell Biochem. 2019 Aug;458(1-2):143-157. doi: 10.1007/s11010-019-03538-3. Epub 2019 Apr 19.
MicroRNAs (miRNAs) regulate osteogenic differentiation of bone cells, which has applications in orthodontics. Here we evaluated the miRNA expression profile of MC3T3-E1 osteoblasts under cyclic tensile stress with chip technology and found that miR-132-3p was up-regulated by 12% cyclic tensile stress. Alkaline phosphatase activity and osteocalcin expression in MC3T3-E1 cells were decreased under these conditions. Smad2 and Smad5 were identified as potential target genes of miR-132-3p. Native and phosphorylated Smad2 and Smad5 expression was negatively correlated with miR-132-3p levels in the cells under cyclic stretch; however, only Smad5 protein level was reduced upon miR-132-3p overexpression. The luciferase reporter assay confirmed a direct interaction between miR-132-3p and Smad5. Thus, miR-132-3p maybe regulates osteoblast differentiation via Smad5 in response to cyclic tensile stress.
微小 RNA（miRNAs）调控着骨细胞的成骨分化，这在正畸学中有应用。在这里，我们利用芯片技术评估了 MC3T3-E1 成骨细胞在循环拉伸应力下的 miRNA 表达谱，发现 miR-132-3p 被 12%的循环拉伸应力上调。在这些条件下，MC3T3-E1 细胞中的碱性磷酸酶活性和骨钙素表达下降。Smad2 和 Smad5 被鉴定为 miR-132-3p 的潜在靶基因。在循环拉伸下，细胞中 miR-132-3p 的表达与天然和磷酸化 Smad2 和 Smad5 的表达呈负相关；然而，只有 Smad5 蛋白水平在 miR-132-3p 过表达时降低。荧光素酶报告基因检测证实了 miR-132-3p 与 Smad5 之间的直接相互作用。因此，miR-132-3p 可能通过 Smad5 来调节成骨细胞分化，以响应循环拉伸应力。
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