Yan Xin, Chen You-Rong, Song Yi-Fan, Yang Meng, Ye Jing, Zhou Gang, Yu Jia-Kuo
Knee Surgery Department of the Institute of Sports Medicine, Peking University Third Hospital, Beijing, China.
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, China.
Front Pharmacol. 2020 Jan 14;10:1534. doi: 10.3389/fphar.2019.01534. eCollection 2019.
Significant progress in osteochondral tissue engineering has been made for biomaterials designed to deliver growth factors that promote tissue regeneration. However, due to diffusion characteristics of hydrogels, the accurate delivery of signaling molecules remains a challenge. In comparison to the direct delivery of growth factors, gene therapy can overcome these challenges by allowing the simultaneous delivery of growth factors and transcription factors, thereby enhancing the multifactorial processes of tissue formation. Scaffold-based gene therapy provides a promising approach for tissue engineering through transfecting cells to enhance the sustained expression of the protein of interest or through silencing target genes associated with bone and joint disease. Reports of the efficacy of gene therapy to regenerate bone/cartilage tissue regeneration are widespread, but reviews on osteochondral tissue engineering using scaffold-based gene therapy are sparse. Herein, we review the recent advances in gene therapy with a focus on tissue engineering scaffolds for osteochondral regeneration.
在用于递送促进组织再生的生长因子的生物材料方面，骨软骨组织工程已取得显著进展。然而，由于水凝胶的扩散特性，信号分子的精确递送仍然是一个挑战。与直接递送生长因子相比，基因治疗可以通过同时递送生长因子和转录因子来克服这些挑战，从而增强组织形成的多因素过程。基于支架的基因治疗通过转染细胞以增强目的蛋白的持续表达或通过沉默与骨和关节疾病相关的靶基因，为组织工程提供了一种有前景的方法。关于基因治疗促进骨/软骨组织再生疗效的报道很普遍，但对基于支架的基因治疗用于骨软骨组织工程的综述却很少。在此，我们综述了基因治疗的最新进展，重点是用于骨软骨再生的组织工程支架。
