聚乙二醇诱导小鼠补体激活后形成的溶血前复合物的研究。

Study on the pre-haemolytic complex formed upon polyethylene glycol-induced activation of mouse complement.

作者信息

Beekhuizen H, van Dijk H, Willers J M

出版信息

Immunol Lett. 1986 Nov 17;14(1):69-73. doi: 10.1016/0165-2478(86)90023-4.

Abstract

The stability and nature of haemolytic activity generated in normal mouse serum upon precipitation with a critical amount of polyethylene glycol and incubation of the redissolved precipitate in the presence of 10 mM EDTA were investigated. The activity appeared to be stable at 0 degrees C which enabled further analysis. The haemolytic mixture was subfractionated by repeated differential polyethylene glycol precipitation yielding two preparations with little or no haemolytic activity by themselves, but which regained activity upon recombination. Material precipitating between 4 and 9% polyethylene glycol became haemolytic when combined with C5-deficient serum. The ultracentrifugation profile of this subfraction strongly suggested the presence of C56-complexes.

摘要

研究了用临界量的聚乙二醇沉淀正常小鼠血清并在10 mM乙二胺四乙酸存在下将重新溶解的沉淀物孵育后产生的溶血活性的稳定性和性质。该活性在0℃似乎是稳定的，这使得能够进行进一步分析。通过重复的聚乙二醇差异沉淀对溶血混合物进行亚分级分离，得到两种自身几乎没有或没有溶血活性但重组后恢复活性的制剂。在4%至9%聚乙二醇之间沉淀的物质与C5缺陷血清结合时会溶血。该亚分级部分的超速离心图谱强烈表明存在C56复合物。