通过一种新的检测方法——聚乙二醇沉淀-补体消耗试验（PEG-CC）检测免疫复合物。
Detection of immune complexes by a new assay, the polyethylene glycol precipitation-complement consumption test (PEG-CC).
作者信息
Harkiss G D, Brown D L
出版信息
Clin Exp Immunol. 1979 Apr;36(1):117-29.
A new assay for the detection of circulating immune complexes, the polyethylene glycol precipitation-complement consumption assay (PEG-CC), is described. The test is both simple and sensitive, and exhibits a high degree of specificity. Immune complexes are first isolated from serum by precipitation in 2.5% polyethylene glycol (PEG) and concentrated. They are then assayed functionally by measuring their ability to fix complement using a sensitive kinetic assay for total haemolytic complement. The test can detect aggregated IgG in serum at concentrations around 6.0 micrograms/ml (about 2.0 micrograms absolute. Using DNA-anti-DNA and ovalbumin-anti-ovalbumin immune complexes prepared in vitro, antigen concentrations less than 0.5 micrograms/ml can be detected. Interference by endogenous complement, polyanions and other factors in test sera has been virtually eliminted by the design of the assay. The increased specificity of the PEG-CC test for immune complexes, should prove useful in the diagnosis and monitoring of immune complex-mediated diseases.
本文描述了一种用于检测循环免疫复合物的新方法——聚乙二醇沉淀-补体消耗试验（PEG-CC）。该试验既简单又灵敏，且具有高度特异性。首先通过在2.5%聚乙二醇（PEG）中沉淀从血清中分离出免疫复合物并进行浓缩。然后通过使用灵敏的总溶血补体动力学测定法测量其固定补体的能力来对其进行功能测定。该试验能够检测血清中浓度约为6.0微克/毫升（约2.0微克绝对值）的聚集IgG。使用体外制备的DNA-抗DNA和卵清蛋白-抗卵清蛋白免疫复合物，可检测到浓度低于0.5微克/毫升的抗原。通过该试验的设计，几乎消除了试验血清中内源性补体、聚阴离子和其他因素的干扰。PEG-CC试验对免疫复合物的特异性提高，应有助于免疫复合物介导疾病的诊断和监测。
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