CAST 基因多态性与两个绵羊合成系胴体价值和肉质的相关性研究。
Association of CAST gene polymorphism with carcass value and meat quality in two synthetic lines of sheep.
机构信息
Institute of Animal Breeding and Biodiversity Conservation, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland.
Institute of Animal Breeding and Biodiversity Conservation, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland.
出版信息
Meat Sci. 2019 Aug;154:69-74. doi: 10.1016/j.meatsci.2019.04.007. Epub 2019 Apr 9.
The aim of study was to estimate genotype frequencies of the polymorphism in the intron 12 of CAST gene of growing lambs, and to evaluate associations between the CAST genotype and carcass and meat traits. A total number of 317 rams representing the synthetic BCP (163) and SCP (154) meaty lines were genotyped by the RFLP/MspI/NcoI/Hin6I. Three genotypes including "aa", "ae" and "ac" with most frequencies of 0.48, 0.27 and 0.19, were observed in whole population. Lambs with the "aa" CAST genotype showed the highest muscle percentage and the lowest fat percentage in the hind legs. On the other hand, the loins of rams with "ac" genotype had the highest intramuscular fat content, indicative of beneficial health-related properties and technological usefulness. However, differences between CAST genotype stated for the sensory properties, texture and thawing and cooking losses of the meat were insignificant. Breeding efforts aimed at the improvement of lamb meat quality should prioritize the selection of the "ac" genotype.
本研究旨在评估生长绵羊 CAST 基因内含子 12 多态性的基因型频率，并评估 CAST 基因型与胴体和肉质性状之间的关联。通过 RFLP/MspI/NcoI/Hin6I，对代表合成 BCP（163）和 SCP（154）肉用系的 317 只公羊进行了基因分型。在整个群体中观察到三种基因型，包括“aa”、“ae”和“ac”，其频率最高分别为 0.48、0.27 和 0.19。具有“aa”CAST 基因型的羔羊后腿肌肉百分比最高，脂肪百分比最低。另一方面，“ac”基因型公羊的腰肉肌内脂肪含量最高，表明具有有益的健康相关特性和技术用途。然而，CAST 基因型对肉的感官特性、质地、解冻和烹饪损失的差异并不显著。旨在提高羊肉质量的育种工作应优先选择“ac”基因型。
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