评估 GH1、CAPN1 和 CAST 多态性作为印度瘤牛和印度瘤牛-普通牛杂交肉牛胴体和肉质性状的标记。

Assessment of GH1, CAPN1 and CAST polymorphisms as markers of carcass and meat traits in Bos indicus and Bos taurus-Bos indicus cross beef cattle.

机构信息

Departamento de Melhoramento e Nutrição Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, SP 18618-000, Brazil.

出版信息

Meat Sci. 2010 Dec;86(4):915-20. doi: 10.1016/j.meatsci.2010.07.016. Epub 2010 Jul 25.

DOI:10.1016/j.meatsci.2010.07.016

PMID:20709461

Abstract

The objective of this study was to investigate the effects of bovine GH1, CAPN1 and CAST gene polymorphisms on carcass and meat traits in Nellore and Nellore x Bos taurus beef cattle. Three hundred animals were genotyped for GH1/MspI (TC/G in intron 3), CAPN316 (AF_252504.2:g.5709C>G) and CAST/RsaI (AY_008267.1:g282C>G) and phenotyped for rib eye area, backfat thickness, intramuscular fat, shear force (SF), and myofibrillar fragmentation index (MFI). No significant associations were observed between the GH1/MspI and CAST/RsaI polymorphisms and phenotypes, although the relation between the CAST/RsaI genotypes and meat tenderness evaluated by MFI approached significant. The fact that the CAPN316 polymorphism did not show adequate segregation in Nellore cattle confirms the difficulty of using this marker in breeding programs of different Bos indicus breeds. However, the positive results of the association analysis obtained for Nellore x B. taurus crosses contributed to the validation of previous findings.

摘要

本研究旨在探讨牛 GH1、CAPN1 和 CAST 基因多态性对尼里-拉菲水牛和尼里-拉菲水牛与瘤牛杂交牛胴体和肉质特性的影响。对 300 只动物进行了 GH1/MspI（TC/G 在 3 号内含子）、CAPN316（AF_252504.2：g.5709C>G）和 CAST/RsaI（AY_008267.1：g282C>G）的基因分型，并对眼肌面积、背膘厚度、肌内脂肪、剪切力（SF）和肌原纤维碎片化指数（MFI）进行了表型分析。尽管 CAST/RsaI 基因型与通过 MFI 评估的肉嫩度之间的关系接近显著，但 GH1/MspI 和 CAST/RsaI 多态性与表型之间未观察到显著关联。CAPN316 多态性在尼里-拉菲牛中未显示出充分的分离，这证实了在不同印度瘤牛品种的育种计划中使用该标记的困难。然而，对尼里-拉菲牛与瘤牛杂交牛获得的关联分析的积极结果有助于验证先前的发现。