Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, 350108, China; The Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou, 350108, China.
Department of Medical Laboratory, Weifang Medical University, Weifang, 261053, China.
Anal Chim Acta. 2019 Aug 13;1065:21-28. doi: 10.1016/j.aca.2019.03.044. Epub 2019 Mar 27.
This report outlines an ultrasensitive electrochemiluminescence (ECL) sensing platform based on water-soluble tungsten oxide quantum dots (WO QDs) for the detection of dopamine (DA) released from P12 cells. The WO QDs with good stability and water solubility were prepared by a facile and green hydrothermal method, and used to modify a glassy carbon electrode (WO QDs/GCE). The proposed ECL sensor exhibited a stable and strong cathodic ECL signal when potassium peroxodisulfate (KSO) as the coreactant in aqueous solution. The possible ECL mechanism was studied and deduced, and the ECL response signal of the proposed sensor decreased rapidly in the presence of dopamine (DA). Under optimal conditions, the ECL signals of WO QDs linearly decreased with the increase of DA concentration in the range of 10 M to 10 M and 10 M to 10 M, respectively. The detection limit was as low as 10 M (S/N = 3). Based on these results, this method has been successfully applied to the determination of DA released by PC12 cells. The detection linear range for the detection of DA released by PC12 cells was from 0.1 to 0.9 μM with a detection limit of 0.045 μM. Therefore, the proposed ECL sensor displayed high sensitivity, good specificity and long-term stability, which may shed light on a new way to construct other high-performance ECL detection systems based on WO or WO QDs-based nanocomposites.
本报告概述了一种基于水溶性氧化钨量子点（WO QDs）的超灵敏电化学发光（ECL）传感平台，用于检测 P12 细胞释放的多巴胺（DA）。通过简便、绿色的水热法制备了具有良好稳定性和水溶性的 WO QDs，并将其用于修饰玻碳电极（WO QDs/GCE）。当过硫酸钾（KSO）作为水溶液中的共反应物时，所提出的 ECL 传感器表现出稳定且强烈的阴极 ECL 信号。研究并推导了可能的 ECL 机制，并且在存在多巴胺（DA）的情况下，所提出的传感器的 ECL 响应信号迅速降低。在最佳条件下，WO QDs 的 ECL 信号在线性范围内分别随 DA 浓度在 10⁻⁶ M 至 10⁻⁵ M 和 10⁻⁵ M 至 10⁻⁴ M 范围内的增加而迅速降低。检测限低至 10⁻⁶ M（S/N = 3）。基于这些结果，该方法已成功应用于测定 PC12 细胞释放的 DA。用于检测 PC12 细胞释放的 DA 的检测线性范围为 0.1 至 0.9 μM，检测限为 0.045 μM。因此，所提出的 ECL 传感器表现出高灵敏度、良好的特异性和长期稳定性，这可能为基于 WO 或 WO QDs 基纳米复合材料构建其他高性能 ECL 检测系统提供新途径。
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