Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.
Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Hubei University, Wuhan 430062, China; Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.
Biosens Bioelectron. 2016 May 15;79:561-7. doi: 10.1016/j.bios.2015.12.098. Epub 2015 Dec 29.
This paper develops a new approach to enhance the electrochemiluminescence (ECL) emission of the Ru(bpy)3(2+)-tripropyl amine (TPrA) system for ultrasensitive determination of ochratoxin A (OTA). Ru(bpy)3(2+)-doped silica nanoparticles (RuSi NPs) act as ECL materials, which are immobilized on the surface of electrode by chitosan to fabricate a solid-state ECL sensor. CdTe quantum dots (QDs) can enhance the ECL emission of the Ru(bpy)3(2+)-TPrA ECL system by energy transfer. This strategy can improve the sensitivity of the sensor. In this assay, we combine the ECL with molecular imprinting technique to improve the selectivity of this sensor. The template molecule could be eluted from the molecularly imprinted polymer (MIP), and the formed cavities could then selectively recognize the target. The cavities could also work as the tunnel for the transfer of coreactant TPrA to produce responsive signal. With the increase of the concentration of OTA in samples, more cavities were filled because of the rebinding of OTA to the MIP surface, resulting in a gradual decrease in ECL intensity. The results showed that the ECL decrease value depended linearly on the logarithm of the OTA concentration in the range from 1.00×10(-5) to 11.13 ng mL(-1) with lower detection limit of 3.0 fg mL(-1) (S/N=3). This ECL sensor has also been applied to detect OTA concentration in the real samples with satisfied results, and the recoveries range from 85.1% to 107.9%.
本文提出了一种新方法，可增强 Ru(bpy)3(2+)-三丙胺（TPrA）电化学发光（ECL）体系的发光强度，用于超灵敏测定赭曲霉毒素 A（OTA）。Ru(bpy)3(2+)掺杂的硅纳米粒子（RuSi NPs）可用作 ECL 材料，通过壳聚糖固定在电极表面，制备固态 ECL 传感器。碲化镉量子点（QDs）可通过能量转移增强 Ru(bpy)3(2+)-TPrA ECL 体系的 ECL 发光。该策略可以提高传感器的灵敏度。在该测定中，我们将 ECL 与分子印迹技术相结合，以提高传感器的选择性。模板分子可从分子印迹聚合物（MIP）中洗脱出来，形成的空腔随后可以特异性地识别目标物。空腔还可以作为核心反应物 TPrA 转移的通道，产生响应信号。随着样品中 OTA 浓度的增加，由于 OTA 与 MIP 表面的再结合，更多的空腔被填充，导致 ECL 强度逐渐降低。结果表明，ECL 降低值与 OTA 浓度的对数在 1.00×10(-5)至 11.13 ng mL(-1)范围内呈线性关系，检测限低至 3.0 fg mL(-1)（S/N=3）。该 ECL 传感器还已应用于实际样品中 OTA 浓度的检测，回收率范围为 85.1%至 107.9%。
