通过分子检测对口腔癌新分类中基于 HPV 状态的头颈部癌症活检标本进行 HPV 检测和基因分型。
HPV detection and genotyping of head and neck cancer biopsies by molecular testing with regard to the new oropharyngeal squamous cell carcinoma classification based on HPV status.
机构信息
Laboratoire de virologie, Hôpital Européen Georges Pompidou, and Assistance Publique - Hôpitaux de Paris, Paris, France.
Département d'Informatique Médicale, Biostatistiques et Santé Publique, Hôpital Européen Georges Pompidou, and Assistance Publique - Hôpitaux de Paris, Paris, France; Faculté de Médecine Paris Descartes, Université Paris Descartes (Paris V), Sorbonne Paris Cité, Paris, France.
出版信息
Pathology. 2019 Jun;51(4):421-425. doi: 10.1016/j.pathol.2019.02.002. Epub 2019 Apr 17.
Recently, both the World Health Organization/International Agency for Research on Cancer (WHO/IARC) and the American Joint Committee on Cancer (AJCC) have classified oropharyngeal squamous cell carcinoma (OPSCC) on the basis of HPV status. For this purpose, the WHO/IARC recommended direct molecular HPV testing. In practice, formalin-fixed, paraffin-embedded (FFPE) biopsy specimens are frequently the only available samples. We herein compared in parallel two commercially available molecular assays that were first designed for cervical HPV detection and genotyping: Inno-Lipa HPV Genotyping Extra II (IL) and Anyplex II HPV28 (AP28). A total of 55 samples were tested. By IL assay, chosen as reference assay, 27 (49.1%) biopsies were positive for HPV16, 10 (18.2%) were positive for HPV but negative for HPV16, and 18 (32.7%) were negative for HPV. A valid result with AP28 was obtained for 51 biopsy samples (92.7%). Among 37 HPV positive samples by IL, 33 (89.2%) were positive by AP28. The agreement between both assays was good (Cohen's κ = 0.78). Among the six discrepancies between assays, always associated with low HPV16 viral load, four biopsies positive for HPV16 by IL could not be detected by AP28. Taken together, these observations demonstrate that both assays could be used in routine HPV detection and genotyping on FFPE biopsy samples of head and neck tumours.
最近，世界卫生组织/国际癌症研究机构（WHO/IARC）和美国癌症联合委员会（AJCC）都根据 HPV 状态对口咽鳞状细胞癌（OPSCC）进行了分类。为此，WHO/IARC 建议直接进行分子 HPV 检测。实际上，福尔马林固定、石蜡包埋（FFPE）活检标本通常是唯一可用的样本。我们在此平行比较了两种最初设计用于检测和 HPV 基因分型的商用分子检测方法：Inno-Lipa HPV Genotyping Extra II（IL）和 Anyplex II HPV28（AP28）。共检测了 55 个样本。通过 IL 检测，选择作为参考检测方法，27 个（49.1%）活检样本 HPV16 阳性，10 个（18.2%）HPV 阳性但 HPV16 阴性，18 个（32.7%）HPV 阴性。AP28 获得了 51 个活检样本的有效结果（92.7%）。在 IL 检测的 37 个 HPV 阳性样本中，AP28 检测到 33 个（89.2%）阳性。两种检测方法之间的一致性良好（Cohen's κ=0.78）。在两种检测方法之间的六个差异中，始终与 HPV16 病毒载量低有关，IL 检测 HPV16 阳性的 4 个活检样本无法被 AP28 检测到。总之，这些观察结果表明，这两种检测方法都可用于头颈部肿瘤 FFPE 活检样本的常规 HPV 检测和基因分型。
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