Vinciguerra Alessandro, Bedarida Vincent, Pronier Charlotte, El Zein Sophie, Wassef Michel, Atallah Sarah, Chatelet Florian, Molher Joffrey, Manivet Philippe, Herman Philippe, Adle-Biassette Homa, Verillaud Benjamin
Otorhinolaryngology and Skull Base Center, AP-HP, Hôpital Lariboisière, 75010 Paris, France.
Centre de Ressources Biologiques Biobank Lariboisière (BB-0033-00064), DMU BioGem, AP-HP, 75010 Paris, France.
J Pers Med. 2023 Apr 29;13(5):767. doi: 10.3390/jpm13050767.
(1) Background: In head and neck squamous cell carcinoma, tumor hypoxia has been associated with radio/chemoresistance and poor prognosis, whereas human papillomavirus (HPV)-positive status has a positive impact on treatment response and survival outcomes. The aim of this study was to evaluate the expression and the potential prognostic value of hypoxia-induced endogenous markers in patients treated for squamous cell carcinoma of the nasal cavity and paranasal sinuses (SNSCC), and their correlation with HPV status. (2) Methods: In this monocentric study, patients treated in a curative intent for a SNSCC were screened retrospectively. Protein expression of CA-IX, GLUT-1, VEGF, VEGF-R1, and HIF-1α was determined by immunohistochemical staining, scored, and then correlated with overall survival (OS) and locoregional recurrence free survival (LRRFS). HPV status was assessed and correlated with hypoxic markers. (3) Results: 40 patients were included. A strong expression of CA-IX, GLUT-1, VEGF, and VEGF-R1 was detected in 30%, 32.5%, 50%, and 37.5% of cases, respectively. HIF-1α was detected in 27.5% of cases. High CA-IX expression was associated in univariate analysis with poor OS ( = 0.035), but there was no significant association between GLUT-1, VEGF, VEGF-R1, and HIF-1α expression, and OS/LRRFS. There was no correlation found between HPV status and hypoxia-induced endogenous markers (all > 0.05). (4) Conclusions: This study provides data on the expression of hypoxia-induced endogenous markers in patients treated for SNSCC and underlines the potential role of CA-IX as a prognostic biomarker for SNSCC.
(1) 背景：在头颈部鳞状细胞癌中，肿瘤缺氧与放射/化学抗性及不良预后相关，而人乳头瘤病毒（HPV）阳性状态对治疗反应和生存结果有积极影响。本研究的目的是评估鼻腔和鼻窦鳞状细胞癌（SNSCC）患者中缺氧诱导的内源性标志物的表达及其潜在预后价值，以及它们与HPV状态的相关性。(2) 方法：在这项单中心研究中，对接受根治性治疗的SNSCC患者进行回顾性筛查。通过免疫组织化学染色测定CA-IX、GLUT-1、VEGF、VEGF-R1和HIF-1α的蛋白表达，进行评分，然后与总生存期（OS）和无局部区域复发生存期（LRRFS）相关联。评估HPV状态并与缺氧标志物相关联。(3) 结果：纳入40例患者。分别在30%、32.5%、50%和37.5%的病例中检测到CA-IX、GLUT-1、VEGF和VEGF-R1的强表达。在27.5%的病例中检测到HIF-1α。单因素分析显示，高CA-IX表达与不良OS相关（P = 0.035），但GLUT-1、VEGF、VEGF-R1和HIF-1α表达与OS/LRRFS之间无显著关联。未发现HPV状态与缺氧诱导的内源性标志物之间存在相关性（所有P>0.05）。(4) 结论：本研究提供了SNSCC患者中缺氧诱导的内源性标志物表达的数据，并强调了CA-IX作为SNSCC预后生物标志物的潜在作用。
