Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung University of Science and Technology, No. 142, Hai-Chuan Rd., Nan-Tzu, Kaohsiung, 811, Taiwan.
College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung, 402, Taiwan.
Arch Microbiol. 2019 Sep;201(7):919-926. doi: 10.1007/s00203-019-01657-5. Epub 2019 Apr 20.
Strain KYPC3, isolated from a freshwater stream in Taiwan, was characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KYPC3 belonged to the genus Rheinheimera. Strain KYPC3 exhibited 16S rRNA gene sequence similarity values of 94.8-97.9% to the type strains of species of the genus Rheinheimera. Strain KYPC3 was most closely related to Rheinheimera chironomi K19414 with 16S rRNA gene sequence similarity of 97.9%. Cells of strain KYPC3 were Gram-stain negative, aerobic, motile by means of a single-polar flagellum, non-spore forming, coccoid or short rods surrounded by a thick capsule and forming off-white coloured colonies. Growth occurred at 15-30 °C (optimum, 20-25 °C), at pH 6-8 (optimum, pH 7) and with 0-0.5% NaCl (optimum, 0%). The major fatty acids (> 10%) of strain KYPC3 were C 3-OH, summed feature 3 (Cω7c and/or Cω6c) and C. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, an uncharacterized aminophospholipid, an uncharacterized phospholipid and an uncharacterized lipid. The major isoprenoid quinone was Q-8. The draft genome was approximately 4.75 Mb in size with a G + C content of 49.8 mol%. The DNA-DNA relatedness of strain KYPC3 with respect to recognized species of the genus Rheinheimera was significantly less than 70%. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain KYPC3 should be classified as a novel species of the genus Rheinheimera, for which the name Rheinheimera riviphila sp. nov. is presented. The type strain is KYPC3 (= BCRC 81008 = LMG 29729 = KCTC 52440).
菌株 KYPC3 是从台湾的一条淡水溪流中分离出来的，采用多相分类学方法进行了特征描述。基于 16S rRNA 基因序列的系统发育分析表明，该菌株属于莱茵海默菌属。菌株 KYPC3 与莱茵海默菌属种的模式菌株的 16S rRNA 基因序列相似性值为 94.8-97.9%。菌株 KYPC3 与 Rheinheimera chironomi K19414 的 16S rRNA 基因序列相似性最高，为 97.9%。菌株 KYPC3 的细胞革兰氏染色阴性，需氧，通过单一极鞭毛运动，不产芽孢，呈球菌或短杆状，被厚荚膜包围，形成灰白色菌落。生长发生在 15-30°C（最佳，20-25°C），pH 值为 6-8（最佳，pH 值为 7），0-0.5%NaCl（最佳，0%）。菌株 KYPC3 的主要脂肪酸（>10%）为 C 3-OH、总和特征 3（Cω7c 和/或 Cω6c）和 C。极性脂谱由磷脂酰乙醇胺、磷脂酰甘油、未鉴定的氨基磷脂、未鉴定的磷脂和未鉴定的脂组成。主要的异戊二烯醌为 Q-8。基因组草图大小约为 4.75 Mb，G+C 含量为 49.8 mol%。菌株 KYPC3 与莱茵海默菌属已鉴定种的 DNA-DNA 相关度显著小于 70%。基于表型和基因型特性以及系统发育推断，菌株 KYPC3 应归类为莱茵海默菌属的一个新种，命名为 Rheinheimera riviphila sp. nov.。模式菌株为 KYPC3（=BCRC 81008=LMG 29729=KCTC 52440）。
