Sheu Shih-Yi, Chen Tzu-Ying, Young Chiu-Chung, Chen Wen-Ming
1Department of Marine Biotechnology, National Kaohsiung University of Science and Technology, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
2Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung University of Science and Technology, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Int J Syst Evol Microbiol. 2019 Jun;69(6):1606-1612. doi: 10.1099/ijsem.0.003365. Epub 2019 Mar 25.
A novel bacterial strain, designated THS-13, isolated from a freshwater mesocosm in Taiwan, was characterized by taking a polyphasic taxonomic approach. Cells of strain THS-13 were Gram-stain-negative, aerobic, rod-shaped, motile by means of a single polar flagellum and formed translucent white coloured colonies. Growth occurred at 20-35 °C (optimum, 25 °C), at pH 5-8 (pH 6) and with 0-2 % NaCl (1 %). Phylogenetic analyses based on 16S rRNA gene and coding sequences of 92 protein clusters revealed that the strain belonged to the family Nevskiaceae in the class Gammaproteobacteria and represented an independent taxon separated from other genera. Strain THS-13 shared low level of 16S rRNA gene sequence similarity (less than 93.0 %) to members of other genera in the family Nevskiaceae and was most closely related to Nevskia aquatilis F2-63 (92.9 %). Strain THS-13 showed less than 73.4 % average nucleotide identity and less than 23.8 % digital DNA-DNA hybridization identity compared to the type strains of related genera within the family Nevskiaceae. The predominant fatty acids were C18 : 1ω7c, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C16 : 0. The major isoprenoid quinone was Q-8 and the DNA G+C content was 67.6 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, an uncharacterized aminophospholipid, an uncharacterized phospholipid and an uncharacterized aminolipid. On the basis of the genotypic and phenotypic data, strain THS-13 represents a novel species of a new genus in the family Nevskiaceae, for which we propose the name Stagnimonas aquatica gen. nov., sp. nov. The type strain is THS-13 (=BCRC 81158=LMG 30925=KCTC 62868).
从台湾的一个淡水微宇宙中分离出的一株新型细菌菌株，命名为THS-13，采用多相分类法对其进行了特征描述。THS-13菌株的细胞革兰氏染色阴性，好氧，杆状，通过单根极生鞭毛运动，形成半透明的白色菌落。在20-35°C（最适温度为25°C）、pH值为5-8（pH值为6）和0-2% NaCl（1%）的条件下生长。基于16S rRNA基因和92个蛋白质簇的编码序列进行的系统发育分析表明，该菌株属于γ-变形菌纲中的涅夫斯基菌科，代表了一个与其他属分离的独立分类单元。THS-13菌株与涅夫斯基菌科其他属成员的1%6S rRNA基因序列相似性较低（低于93.0%），与水生涅夫斯基菌F2-63关系最为密切（92.9%）。与涅夫斯基菌科相关属的模式菌株相比，THS-13菌株的平均核苷酸同一性低于73.4%，数字DNA-DNA杂交同一性低于23.8%。主要脂肪酸为C18:1ω%，总和特征3（C16:1ω%和/或C16:1ω%）和异C16:0。主要类异戊二烯醌为Q-8，DNA G+C含量为67.6 mol%。主要极性脂质为磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的氨基磷脂、一种未鉴定的磷脂和一种未鉴定的氨基脂质。基于基因型和表型数据，THS-13菌株代表了涅夫斯基菌科一个新属的一个新物种，我们将其命名为水生停滞单胞菌（Stagnimonas aquatica），新属，新种。模式菌株为THS-13（=BCRC 81158=LMG 30925=KCTC 62868）。
