Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Sugarcane Research Center, Chinese Academy of Agricultural Science, Nanning, 530007, China.
Mol Biol Rep. 2019 Aug;46(4):3777-3789. doi: 10.1007/s11033-019-04820-9. Epub 2019 Apr 20.
Fusarium verticillioides is the pathogen associated with pokkah boeng disease (PBD), the most significant airborne disease of sugarcane. The molecular mechanisms that regulate the defense responses of sugarcane towards this fungus are not yet fully known. Samples of 'YT 94/128' (resistant, R) and 'GT 37' (susceptible, S) inoculated with F. verticillioides on the 14 days post-inoculation were used to analyze the transcriptome to screen R genes. In total, 80.93 Gb of data and 76,175 Unigenes were obtained after assembling the sequencing data, and comparisons of Unigenes with NR, Swiss-prot, KOG, and KEGG databases confirmed 42,451 Unigenes. The analysis of differentially expression genes (DEGs) in each sample revealed 9092 DEGs in 'YT 94/128,' including 8131 up-regulated DEGs and 961 down-regulated DEGs; there were 9829 DEGs in 'GT 37,' including 7552 up-regulated DEGs and 2277 down-regulated DEGs. The identified DEGs were mainly involved in catalytic enzyme activity, cell protease, hydrolytic enzymes, peptide enzyme, protein metabolism process of negative regulation, phenylpropanoid metabolism, extracellular region, aldehyde dehydrogenase, endopeptidase, REDOX enzyme, protein kinases, and phosphoric acid transferase categories. KEGG pathway clustering analysis showed that the DEGs involved in resistance were significantly related to metabolic pathways of phenylpropanoid biosynthesis, cutin, suberine and wax biosynthesis, nitrogenous metabolism, biosynthesis of secondary metabolites, and plant-pathogen interactions. This application of transcriptomic data clarifies the mechanism of interactions between sugarcane and F. verticillioides, which can help to reveal disease-related metabolic pathways, molecular regulatory networks, and key genes involved in sugarcane responses to F. verticillioides.
镰刀菌是与甘蔗最主要的气传病害——菠萝心腐病（PBD）相关的病原体。然而，调控甘蔗针对该真菌防御反应的分子机制尚未完全明晰。本研究采用接种镰刀菌的‘YT 94/128’（抗性，R）和‘GT 37’（敏感，S）样本，进行转录组分析以筛选 R 基因。组装测序数据后，共获得 80.93GB 的数据和 76175 条 Unigenes，与 NR、Swiss-prot、KOG 和 KEGG 数据库的 Unigenes 比对，共鉴定到 42451 条 Unigenes。对每个样本的差异表达基因（DEGs）进行分析，在‘YT 94/128’中共鉴定到 9092 个 DEGs，其中 8131 个上调 DEGs 和 961 个下调 DEGs；在‘GT 37’中共鉴定到 9829 个 DEGs，其中 7552 个上调 DEGs和 2277 个下调 DEGs。鉴定的 DEGs 主要参与催化酶活性、细胞蛋白酶、水解酶、肽酶、负调控的蛋白质代谢过程、苯丙烷代谢、细胞外区域、醛脱氢酶、内肽酶、氧化还原酶、蛋白激酶和磷酸转移酶等类别。KEGG 通路聚类分析表明，参与抗性的 DEGs 与苯丙烷类生物合成、角质、软木脂和蜡生物合成、氮代谢、次生代谢物合成和植物-病原体相互作用的代谢途径显著相关。本转录组数据的应用阐明了甘蔗与镰刀菌相互作用的机制，有助于揭示与疾病相关的代谢途径、分子调控网络以及甘蔗对镰刀菌反应的关键基因。
