Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences/Sugarcane Research Center, Chinese Academy of Agricultural Science/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture. Nanning, 530007, China; College of Agriculture, State Key Laboratory of Conservation and Utilization of Subtropical Agrobioresources, Guangxi University, Nanning, Guangxi, 530004, China.
Flowers Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, 530007, China.
J Plant Physiol. 2020 Aug;251:153207. doi: 10.1016/j.jplph.2020.153207. Epub 2020 Jun 6.
Pokkah boeng disease (PBD) is a foliar disease causing severe losses in sugarcane crop production. Research into resistance mechanisms against the causal agent, Fusarium verticillioides, is particularly important for farmers and researchers. This work based on the comprehensive analysis of metabolic, proteomic, and bioinformatics data on nitrogen (N) metabolism, which revealed that this biosynthetic reactions was closely related to resistance mechanisms in the sugarcane- F. verticillioides interaction. Our results suggested that pathogen infection reduced the suppression of nitrate reductase (NR) activity, reducing ammonium nitrogen (NH-N) and nitrate nitrogen (NO-N) assimilation, which reduces glutamine synthetase (GS), glutamate synthetase (GOGAT) activity and polynucleotide synthesis and promotes RNA degradation, resulting in a decrease in ribosome levels and protein synthesis. Cysteine was found to be associated with the symptoms of PBD, while alanine, lysine, proline, and glutamic acid were found to be involved in protective and regulatory mechanisms as well. Additionally, glutamate played an important role in sugarcane defense against pathogens through the biosynthesis of proline and polyamines. Cyanamide, glutamate, proline, tyrosine, and arachidonic acid metabolism actively participate in resistance and response to stress. C5XPZ6 and C5XCA6 were considered to be critical proteins and key effectors according to this study. In conclusion, we have identified potential proteins and pathways involved in sugarcane resistance to F. verticillioides, revealing new findings that may be useful in the design of future diagnostics or sugarcane protection strategies and providing new insights into the molecular mechanisms of sugarcane-pathogen interactions.
镰刀菌顶腐病（PBD）是一种叶片病害，会导致甘蔗作物产量严重损失。因此，研究针对病原菌尖孢镰刀菌的抗性机制对农民和研究人员来说尤为重要。本研究基于对氮（N）代谢的代谢组学、蛋白质组学和生物信息学数据的综合分析，结果表明该生物合成反应与甘蔗-尖孢镰刀菌互作中的抗性机制密切相关。我们的研究结果表明，病原菌感染降低了硝酸还原酶（NR）活性的抑制作用，减少了铵态氮（NH-N）和硝态氮（NO-N）的同化，从而降低了谷氨酰胺合成酶（GS）、谷氨酸合成酶（GOGAT）的活性和多核苷酸的合成，并促进了 RNA 的降解，导致核糖体水平和蛋白质合成减少。本研究还发现半胱氨酸与 PBD 的症状有关，而丙氨酸、赖氨酸、脯氨酸和谷氨酸则参与了保护和调节机制。此外，谷氨酸通过脯氨酸和多胺的生物合成在甘蔗抵御病原体方面发挥了重要作用。氰氨酰谷氨酸、脯氨酸、酪氨酸和花生四烯酸代谢积极参与抗性和应激反应。根据本研究，C5XPZ6 和 C5XCA6 被认为是关键蛋白和关键效应子。总之，本研究鉴定了与甘蔗对尖孢镰刀菌抗性相关的潜在蛋白和途径，揭示了新的发现，这些发现可能有助于未来设计诊断或甘蔗保护策略，并为甘蔗-病原菌互作的分子机制提供新的见解。
