Tsujisaki M, Igarashi M, Sakaguchi K, Eisinger M, Herlyn M, Ferrone S
J Immunol. 1987 Feb 15;138(4):1310-6.
The effect of recombinant immune interferon (IFN-gamma) on the expression and shedding of HLA antigens and of melanoma-associated antigens (MAA) by epidermal melanocytes was investigated by using serologic and immunochemical techniques. IFN-gamma enhances the expression and/or shedding of HLA class I antigens and of the cytoplasmic MAA defined by monoclonal antibody (MoAb) 465.12S and induces a slight reduction in the expression of the high m.w. melanoma-associated antigen (HMW-MAA). In agreement with the data in the literature, melanocytes incubated with IFN-gamma acquire HLA-DR, -DQ, and -DP antigens. Contrary to previous information in the literature, the effect is not restricted to HLA class II antigens, since IFN-gamma also induces the expression of the 96-kDa MAA recognized by MoAb CL203. The effect of IFN-gamma on HLA class II antigens and 96-kDa MAA is dose and time dependent and is specific, because recombinant leukocyte interferon affects the expression of neither type of antigen. In spite of the expression of HLA class II antigens, IFN-gamma-treated melanocytes do not acquire the ability to stimulate the proliferation of allogeneic lymphocytes. HLA-DR antigens are more susceptible to induction by IFN-gamma than HLA-DQ and -DP antigens, since the percentage of melanocytes acquiring HLA-DQ and -DP antigens is lower than that acquiring HLA-DR antigens. Furthermore, the dose of IFN-gamma is higher and the time of incubation is longer to induce HLA-DQ and -DP antigens than to induce HLA-DR antigens. The differential susceptibility of HLA-DR, -DQ, and -DP antigens as well as of melanocytes from various donors to the modulating effect of IFN-gamma may provide an explanation for the more frequent detection of HLA-DR than of HLA-DQ and -DP antigens in melanoma lesions and for the expression of HLA class II antigens by some, but not all, melanoma lesions.
采用血清学和免疫化学技术，研究了重组免疫干扰素（IFN-γ）对表皮黑素细胞HLA抗原及黑素瘤相关抗原（MAA）表达和释放的影响。IFN-γ增强了HLA I类抗原以及由单克隆抗体（MoAb）465.12S界定的细胞质MAA的表达和/或释放，并导致高分子量黑素瘤相关抗原（HMW-MAA）的表达略有降低。与文献数据一致，用IFN-γ孵育的黑素细胞获得了HLA-DR、-DQ和-DP抗原。与文献中先前的信息相反，这种作用并不局限于HLA II类抗原，因为IFN-γ还诱导了MoAb CL203识别的96-kDa MAA的表达。IFN-γ对HLA II类抗原和96-kDa MAA的作用具有剂量和时间依赖性且具有特异性，因为重组白细胞干扰素对这两种抗原的表达均无影响。尽管表达了HLA II类抗原，但经IFN-γ处理的黑素细胞并未获得刺激同种异体淋巴细胞增殖的能力。HLA-DR抗原比HLA-DQ和-DP抗原更容易被IFN-γ诱导，因为获得HLA-DQ和-DP抗原的黑素细胞百分比低于获得HLA-DR抗原的黑素细胞百分比。此外，诱导HLA-DQ和-DP抗原所需的IFN-γ剂量更高，孵育时间更长。HLA-DR、-DQ和-DP抗原以及来自不同供体的黑素细胞对IFN-γ调节作用的不同敏感性，可能解释了黑素瘤病变中HLA-DR抗原比HLA-DQ和-DP抗原更频繁被检测到，以及部分而非所有黑素瘤病变表达HLA II类抗原的现象。
