根据羟基酪醇和酪醇衍生物的含量对橄榄油进行标记的简单、绿色工具：使用天然深共熔溶剂进行提取和直接分光光度分析。

An easy and green tool for olive oils labelling according to the contents of hydroxytyrosol and tyrosol derivatives: Extraction with a natural deep eutectic solvent and direct spectrophotometric analysis.

机构信息

Department of Soil, Plant and Food Science, University of Bari Aldo Moro, Via Amendola 165/a, 70126 Bari, Italy.

出版信息

Food Chem. 2019 Sep 1;291:1-6. doi: 10.1016/j.foodchem.2019.03.139. Epub 2019 Mar 27.

DOI:10.1016/j.foodchem.2019.03.139

Abstract

Natural deep eutectic solvents (NADES) are a green, promising class of solvents. Phenolic compounds are among the targets of NADES' use in foods. A health claim is admitted by European Regulations for olive oils (OOs) containing at least 250 mg kg of selected polyphenols (hydroxytyrosol and its derivatives, included tyrosol, HTD), but their determination involves analytical issues that are being currently debated. A NADES based on glucose and lactic acid was here used to develop a green and rapid tool for correct labelling of OOs. 163 OOs were submitted to liquid/liquid extraction with the NADES. Regression and classification approaches were adopted to relate features of the UV spectra of extracts to HTD content. The models allowed to assess HTD content for screening purposes (R = 0.84, RMSEP = 35.5 mg kg). For labelling purposes, oils could be labelled according to health claim limits with an error of 0.6%.

摘要

天然深共晶溶剂 (NADES) 是一类绿色、有前景的溶剂。酚类化合物是 NADES 在食品中应用的目标之一。欧盟法规承认橄榄油 (OO) 中的健康声称，只要其含有至少 250mgkg 的特定多酚（羟基酪醇及其衍生物，包括酪醇、HTD），但它们的测定涉及到目前正在讨论的分析问题。这里使用基于葡萄糖和乳酸的 NADES 来开发一种用于正确标注 OO 的绿色快速工具。将 163 种 OO 进行 NADES 液/液萃取。采用回归和分类方法将提取物的紫外光谱特征与 HTD 含量相关联。这些模型允许对 HTD 含量进行筛选目的的评估（R=0.84，RMSEP=35.5mgkg）。对于标签目的，可以根据健康声称的限制对油进行标记，误差为 0.6%。