Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy.
Department of Biomedical, Metabolical and Neural Sciences, University of Modena and Reggio Emilia, Via del Pozzo 71, 41124 Modena, Italy.
J Chromatogr A. 2019 Jul 19;1597:179-186. doi: 10.1016/j.chroma.2019.03.034. Epub 2019 Apr 3.
Fibre-type Cannabis sativa L. (hemp) represents a valuable resource in many different fields, including both the pharmaceutical and food ones. This plant contains non-psychoactive cannabinoids, a class of bioactive compounds biosynthesized in both female and male inflorescences. Among them, cannabidiol (CBD) is the most interesting compound from a medicinal point of view. Indeed, several scientific studies have proved its therapeutic potential in a large number of pathologies, in addition to its biological effects attributable to its antioxidant, neuroprotective and anti-inflammatory properties. The analysis of the amount of cannabinoids in food and food supplements represents a critical issue in the ambit of both the quality assurance and the dietary intake control of these biologically active compounds. In this ambit, a particular attention is necessary for apiary products, since they are widely consumed and they can be produced by bees starting from different floral sources. In the light of all the above, the aim of this study was to develop for the first time a new analytical method based on RP-HPLC with ESI-MS/MS detection for the determination of CBD and related cannabinoids in honey. A quick, easy, cheap, effective, rugged and safe (QuEChERS) extraction procedure with an un-buffered method was selected and optimised as the more suitable protocol. As regards detection, it was carried out by using a linear ion trap quadrupole (QTRAP) mass analyser, operated in the multiple reaction monitoring (MRM) mode. Hemp male inflorescences and pollen were analysed in parallel by means of HPLC-UV/DAD, since bees can transfer pollen into their hives and, consequently, into beehive products. The method developed and validated for the first time in this work was finally applied to the analysis of cannabinoids in honey samples, thus demonstrating to be a useful tool for both quality control and safety assurance.
纤维型大麻（hemp）是一种在许多领域都有价值的资源，包括医药和食品领域。这种植物含有非精神活性大麻素，这是一类在雌性和雄性花序中生物合成的生物活性化合物。其中，大麻二酚（CBD）是从药用角度来看最有趣的化合物。事实上，许多科学研究已经证明了它在许多疾病中的治疗潜力，此外，它还具有抗氧化、神经保护和抗炎作用。分析食品和食品补充剂中的大麻素含量是质量保证和这些生物活性化合物饮食摄入控制的关键问题。在这个范围内，蜂产品需要特别注意，因为它们被广泛食用，并且可以由蜜蜂从不同的花源中生产。鉴于上述所有情况，本研究的目的是首次开发一种新的基于反相高效液相色谱-电喷雾串联质谱（RP-HPLC-ESI-MS/MS）检测法，用于测定蜂蜜中的 CBD 和相关大麻素。选择并优化了一种快速、简单、廉价、有效、耐用和安全（QuEChERS）的无缓冲提取方法作为更合适的方案。在检测方面，使用线性离子阱四极杆（QTRAP）质谱仪，以多反应监测（MRM）模式进行检测。同时，通过 HPLC-UV/DAD 分析大麻雄性花序和花粉，因为蜜蜂可以将花粉转移到蜂巢中，进而转移到蜂产品中。本研究首次开发并验证了该方法，最后将其应用于蜂蜜样品中大麻素的分析，证明了它是质量控制和安全保证的有用工具。
