Department of Anesthesiology, The Second Hospital of Jilin University, Changchun, Jilin, China.
Department of Hepatopancreatobiliary Medicine, The Second Hospital of Jilin University, Changchun, Jilin, China.
Cancer Biomark. 2019;25(1):115-126. doi: 10.3233/CBM-192399.
Hepatocellular carcinoma (HCC) is one of the most common malignancies, and its global morbidity and mortality are increasing. Previous studies confirmed that miR-342 was involved in the development and progression of malignant tumors. However, the relationship between miR-342 and Wnt/β-catenin signaling pathway in HCC remains unknown.
Cell viability was detected by MTT assay. Immunofluorescence staining was used to detect Brdu-positive cells and Western blot was used to detect the apoptotic proteins. Furthermore, linear correlation analysis was used to investigate the possible relationship between miR-342 and the downstream genes of Wnt/β-catenin signaling pathway in the progression of HCC.
Over-expression of miR-342 significantly reduced cell proliferation and obviously increased apoptosis in HCC, while silencing of miR-342 showed an opposite effect on HCC cell proliferation and apoptosis. In addition, we found that the CXCL12 was the target gene of miR-342. This study also demonstrated that miR-342 up-regulation suppressed Wnt/β-catenin signaling pathway by inhibiting CXCL12 expression.
Up-regulation of miR-342 inhibited cell proliferation and induced cell apoptosis in HCC by inhibiting Wnt/β-catenin signaling pathway, suggesting that miR-342 might act as a promising tumor gene therapeutic target for HCC patients.
肝细胞癌（HCC）是最常见的恶性肿瘤之一，其全球发病率和死亡率正在上升。先前的研究证实 miR-342 参与了恶性肿瘤的发生和发展。然而，miR-342 与 HCC 中的 Wnt/β-catenin 信号通路之间的关系尚不清楚。
通过 MTT 测定法检测细胞活力。免疫荧光染色用于检测 Brdu 阳性细胞，Western blot 用于检测凋亡蛋白。此外，线性相关分析用于研究 miR-342 与 Wnt/β-catenin 信号通路下游基因在 HCC 进展中的可能关系。
miR-342 的过表达显著降低了 HCC 中的细胞增殖，并明显增加了细胞凋亡，而 miR-342 的沉默对 HCC 细胞增殖和凋亡则产生了相反的影响。此外，我们发现 CXCL12 是 miR-342 的靶基因。本研究还表明，miR-342 通过抑制 CXCL12 的表达抑制了 Wnt/β-catenin 信号通路。
miR-342 的上调通过抑制 Wnt/β-catenin 信号通路抑制了 HCC 中的细胞增殖并诱导了细胞凋亡，表明 miR-342 可能作为 HCC 患者有前途的肿瘤基因治疗靶标。
