Moore G R, Mann S, Bannister J V
J Inorg Biochem. 1986 Oct-Nov;28(2-3):329-36. doi: 10.1016/0162-0134(86)80097-6.
A nonheme-iron-containing cytochrome b557, also known as bacterioferritin, was isolated from Pseudomonas aeruginosa. Gel electrophoresis showed that the protein subunits had a molecular weight of 18 kD and it is suggested that the intact molecule contained 24 subunits. The isolated protein contained 8.7% by weight Fe and 8% by weight phosphate. Most of the Fe was contained in the nonheme-iron core and could be readily removed by dialysis against 0.12 M thioglycollic acid. The resulting apobacterioferritin contained approximately one protoporphyrin IX group for every five subunits and, in addition, an unidentified fluorophor. The nonheme-iron core was found to be amorphous with a mean core size of 60-65 A.
一种不含血红素铁的细胞色素b557，也被称为细菌铁蛋白，是从铜绿假单胞菌中分离出来的。凝胶电泳显示该蛋白质亚基的分子量为18 kD，推测完整分子包含24个亚基。分离出的蛋白质含铁量为8.7%（重量），含磷量为8%（重量）。大部分铁存在于非血红素铁核心中，通过用0.12 M巯基乙酸透析可轻易去除。所得的脱辅基细菌铁蛋白每五个亚基大约含有一个原卟啉IX基团，此外还有一种未鉴定的荧光团。发现非血红素铁核心呈无定形，平均核心大小为60 - 65 Å。
