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利用分子和温室嫁接相结合的工具评估非洲选定木薯品种对木薯花叶病的抗性

Evaluation of resistance to cassava mosaic disease in selected African cassava cultivars using combined molecular and greenhouse grafting tools.

作者信息

Houngue Jerome Anani, Zandjanakou-Tachin Martine, Ngalle Hermine Bille, Pita Justin S, Cacaï Gilles Habib Todjro, Ngatat Sergine E, Bell Joseph Martin, Ahanhanzo Corneille

机构信息

Central Laboratory of Plant Biotechnology and Plant Breeding, Department of Genetics and Biotechnology, Faculty of Science and Technique, University of Abomey-Calavi, Benin.

Laboratory of Genetics and Plant Breeding, Department of Plant Biology, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.

出版信息

Physiol Mol Plant Pathol. 2019 Jan;105:47-53. doi: 10.1016/j.pmpp.2018.07.003.

Abstract

Cassava mosaic disease (CMD) threatens cassava () production in Africa. A total of 24 selected cultivars were screened against CMD using combined molecular and greenhouse grafting tools. Disease severity was recorded for 10 weeks after inoculation and the molecular markers associated with were detected by PCR. CMD severity significantly differed ( < 0.0001) among cultivars. Twelve cultivars were morphologically resistant and eight of these possessed and four did not. These results suggest that there are several CMD-resistant cassava cultivars that could be recommended for on-farm production and for conservation and breeding programs.

摘要

木薯花叶病(CMD)威胁着非洲的木薯生产。使用分子和温室嫁接相结合的工具,对总共24个选定的品种进行了抗CMD筛选。接种后10周记录病害严重程度,并通过PCR检测与CMD相关的分子标记。不同品种间CMD严重程度差异显著(P<0.0001)。12个品种在形态上具有抗性,其中8个含有CMD抗性基因,4个不含。这些结果表明,有几个抗CMD的木薯品种可推荐用于农场生产以及保护和育种计划。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0a5/6472601/c00bfa5960b6/gr1.jpg

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