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pH值和盐类对水溶液中溶菌酶和牛血清白蛋白偏摩尔体积的影响

Influence of pH and Salts on Partial Molar Volume of Lysozyme and Bovine Serum Albumin in Aqueous Solutions.

作者信息

Jirasek Fabian, Garcia Edder J, Hackemann Eva, Galeotti Nadia, Hasse Hans

机构信息

University of Kaiserslautern Laboratory of Engineering Thermodynamics (LTD) Erwin-Schrödinger-Strasse 44 67663 Kaiserslautern Germany.

出版信息

Chem Eng Technol. 2018 Dec;41(12):2337-2345. doi: 10.1002/ceat.201800242. Epub 2018 Oct 23.

DOI:10.1002/ceat.201800242
PMID:31007400
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6472598/
Abstract

The partial molar volume of lysozyme and bovine serum albumin in aqueous solutions at different pH values and in aqueous solutions containing sodium chloride, ammonium chloride, sodium sulfate, or ammonium sulfate at different concentrations at pH 7.0 was investigated experimentally at 298.15 K and 1 bar. It was found that the influence of the pH value and the salts on the partial molar volume of the proteins is small, but trends were measurable. Furthermore, the partial molar volume of lysozyme in pure water at different pH values and in aqueous solutions with different sodium chloride concentrations at pH 7.0 was predicted by molecular simulations. The predictions are in good agreement with the experimental data.

摘要

在298.15 K和1巴的条件下，通过实验研究了溶菌酶和牛血清白蛋白在不同pH值的水溶液以及pH值为7.0、含有不同浓度氯化钠、氯化铵、硫酸钠或硫酸铵的水溶液中的偏摩尔体积。结果发现，pH值和盐对蛋白质偏摩尔体积的影响较小，但仍可测量到一定趋势。此外，通过分子模拟预测了溶菌酶在不同pH值的纯水中以及pH值为7.0、含有不同氯化钠浓度的水溶液中的偏摩尔体积。预测结果与实验数据吻合良好。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d6/6472598/d21a7c361f32/CEAT-41-2337-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d6/6472598/7f0da8c1dc72/CEAT-41-2337-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d6/6472598/79fe62c5a3c6/CEAT-41-2337-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d6/6472598/d57f8cb81be0/CEAT-41-2337-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d6/6472598/bee3994eec55/CEAT-41-2337-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d6/6472598/d21a7c361f32/CEAT-41-2337-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d6/6472598/7f0da8c1dc72/CEAT-41-2337-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d6/6472598/79fe62c5a3c6/CEAT-41-2337-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d6/6472598/d57f8cb81be0/CEAT-41-2337-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d6/6472598/bee3994eec55/CEAT-41-2337-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d6/6472598/d21a7c361f32/CEAT-41-2337-g005.jpg

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