Berg K, Aune T
J Toxicol Environ Health. 1987;20(1-2):187-97. doi: 10.1080/15287398709530971.
The acute toxicity of extracts of blue-green algae was tested in freshly prepared rat hepatocytes in suspension. The results were compared with the traditional in vivo mouse bioassay. Sixty samples of natural algal blooms from freshwater lakes in Norway, Sweden, and Finland and 14 samples cultured in the laboratory were tested. The mouse bioassay revealed hepatotoxins in a large number of the algae, while neurotoxins were not found. Acute hepatotoxicity in vitro was scored by measurement of leakage of the enzyme lactate dehydrogenase (LDH) from damaged cells and of morphological changes of the cells. The correlation coefficients between mouse toxicity and LDH, mouse toxicity and morphological cell damage, and between LDH and morphological cell damage were 0.812, 0.735, and 0.882, respectively. Consequently, the rat hepatocyte toxicity test seems to be well suited for screening blooms of blue-green algae for the presence of hepatotoxins.
在新鲜制备的悬浮大鼠肝细胞中测试了蓝藻提取物的急性毒性。将结果与传统的体内小鼠生物测定法进行了比较。对来自挪威、瑞典和芬兰淡水湖的60个天然藻华样本以及在实验室培养的14个样本进行了测试。小鼠生物测定法显示大量藻类中存在肝毒素，而未发现神经毒素。通过测量受损细胞中乳酸脱氢酶（LDH）的泄漏以及细胞的形态变化来对体外急性肝毒性进行评分。小鼠毒性与LDH之间、小鼠毒性与细胞形态损伤之间以及LDH与细胞形态损伤之间的相关系数分别为0.812、0.735和0.882。因此，大鼠肝细胞毒性试验似乎非常适合筛查蓝藻藻华是否存在肝毒素。
