College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
Hunan Co-Innovation Center of Animal Production Safety, Changsha, China.
J Anim Physiol Anim Nutr (Berl). 2019 Jul;103(4):1081-1089. doi: 10.1111/jpn.13094. Epub 2019 Apr 22.
This study was conducted to investigate the effects of dietary supplementation with montmorillonite (MMT) on performance, intestinal endotoxin concentration, gut mucosal oxidation status, intestinal morphology and permeability, and immunological barrier function of laying hens during late production. Four hundred and eighty 75-week-old laying hens (Lohmann Brown) were randomly assigned to five treatments with eight replicates per treatment and 12 hens in each replicate. The hens were fed the basal diet supplemented with 0 (control), 0.3, 0.6, 0.9, or 1.2 g MMT/kg for 70 days. Compared with the control, supplemented with 0.9 g MMT/kg increased egg mass significantly (p < 0.05) during weeks 1-5 of the experiment. Supplemented with 0.6 and 0.9 g MMT/kg also increased the endotoxin concentration in the ileal digesta (p < 0.05), but decreased the MDA concentration in the ileum significantly (p < 0.05). The T-AOC in the jejunum of the group fed 0.3 g MMT/kg was significantly increased (p < 0.05). Compared with the control, the villus height:crypt depth of ileum from the groups fed 0.6, 0.9, and 1.2 g MMT/kg increased significantly (p < 0.05). The sIgA concentration of jejunum in the groups fed 0.6 and 0.9 g MMT/kg was higher (p < 0.05) than the control. The MMT supplementation linearly increased (p < 0.05) the mRNA expression of claudin-1 and claudin-5 in the jejunum. Dietary MMT supplementation down-regulated the mRNA expression of NF-κB P65 and TNF-α in the jejunum in a linear and quadratic manner (p < 0.05). The IL-1β mRNA expression of jejunum in the group fed 0.6 g MMT/kg was lower (p < 0.05) than the control. In conclusion, dietary supplementation with MMT may improve the gut barrier functions and suggests that 0.9 g/kg of MMT in diets may be the optimal supplemental level for laying hens in late production.
本研究旨在探讨日粮中添加蒙脱石（MMT）对产蛋后期母鸡生产性能、肠道内毒素浓度、肠道黏膜氧化状态、肠道形态和通透性以及免疫屏障功能的影响。将 480 只 75 周龄的产蛋母鸡（罗曼褐）随机分为 5 个处理组，每个处理组设 8 个重复，每个重复 12 只母鸡。母鸡饲喂基础日粮，同时添加 0（对照组）、0.3、0.6、0.9 或 1.2 g MMT/kg 日粮，试验持续 70 d。与对照组相比，试验第 1-5 周，添加 0.9 g MMT/kg 组的蛋重显著增加（p<0.05）。添加 0.6 和 0.9 g MMT/kg 组回肠食糜内毒素浓度也显著升高（p<0.05），但显著降低了回肠 MDA 浓度（p<0.05）。添加 0.3 g MMT/kg 组空肠 T-AOC 显著增加（p<0.05）。与对照组相比，添加 0.6、0.9 和 1.2 g MMT/kg 组的回肠绒毛高度/隐窝深度显著增加（p<0.05）。添加 0.6 和 0.9 g MMT/kg 组空肠分泌型免疫球蛋白 A（sIgA）浓度高于对照组（p<0.05）。MMT 线性增加（p<0.05）了空肠中 Claudin-1 和 Claudin-5 的 mRNA 表达。日粮添加 MMT 线性和二次下调（p<0.05）了空肠中 NF-κB P65 和 TNF-α 的 mRNA 表达。添加 0.6 g MMT/kg 组的空肠白细胞介素 1β（IL-1β）mRNA 表达低于对照组（p<0.05）。综上所述，日粮添加 MMT 可以改善肠道屏障功能，提示日粮中添加 0.9 g/kg MMT 可能是产蛋后期母鸡的最佳添加水平。
