Key Laboratory of Agro-ecological Processes in Subtropical Region , Institute of Subtropical Agriculture, Chinese Academy of Sciences , Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha , Hunan 410125 , People's Republic of China.
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine , Nanjing Agricultural University , Nanjing 210095 , People's Republic of China.
J Agric Food Chem. 2019 May 8;67(18):5096-5104. doi: 10.1021/acs.jafc.9b00446. Epub 2019 Apr 29.
Tryptophan (Trp) can produce bioactive compounds for appetite regulation, calcium mobilization, and mammary gland homeostasis via a serotonin pathway. This study evaluated the effects of Trp supplementation on the reproduction performance, milk yield, and composition of lactating sows, growth performance of their piglets, and the secretion function of porcine mammary epithelial cells (PMECs). The infrared emulsion analyzer and ELISA analyses revealed that feeding sows with a 0.12% Trp addition increased ( P < 0.05) sow average daily feed intake, milk yield, milk calcium concentration, average daily gain of piglets, fatty acid synthase (FAS) and lactose synthase (LS), β-casein secretion, intracellular Ca level, the expression of calcium binding protein CaM, and the activity of CaMKII. In a cellular experiment of PMECs treated with Trp, ELISA and flow cytometry analyses revealed that the pretreatment of a Trp hydroxylase inhibitor reduced ( P < 0.05) FAS and LS synthesis, the intracellular Ca level, and the activity of CaMKII. In conclusion, Trp supplementation at 0.12% increased sows' reproductive performance, milk yield, and calcium concentration and piglets' growth performance. Milk yield increased by Trp was linked to 5-hydroxytryptamine-mediated synthesis of FAS, LS, and β-casein in PMECs, while the increase in calcium concentration was attributed to increasing CaM expression and CAMKII activity.
色氨酸（Trp）可以通过 5-羟色胺途径产生生物活性化合物，用于调节食欲、钙动员和乳腺稳态。本研究评估了色氨酸补充对哺乳期母猪繁殖性能、产奶量和组成、仔猪生长性能以及猪乳腺上皮细胞（PMEC）分泌功能的影响。红外乳脂分析仪和 ELISA 分析表明，给母猪饲喂 0.12%的色氨酸可提高（P<0.05）母猪平均日采食量、产奶量、乳钙浓度、仔猪平均日增重、脂肪酸合酶（FAS）和乳糖合酶（LS）、β-酪蛋白分泌、细胞内 Ca 水平、钙结合蛋白 CaM 的表达和 CaMKII 的活性。在 PMECs 用色氨酸处理的细胞实验中，ELISA 和流式细胞术分析表明，色氨酸羟化酶抑制剂预处理可降低（P<0.05）FAS 和 LS 合成、细胞内 Ca 水平和 CaMKII 的活性。总之，0.12%的色氨酸补充提高了母猪的繁殖性能、产奶量和钙浓度以及仔猪的生长性能。色氨酸增加产奶量与 PMECs 中 5-羟色胺介导的 FAS、LS 和 β-酪蛋白合成有关，而钙浓度的增加归因于 CaM 表达和 CaMKII 活性的增加。
