Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, Shandong, China.
Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Agricultural University, Tai'an, 271018, Shandong, China.
Sci Rep. 2024 Mar 27;14(1):7291. doi: 10.1038/s41598-024-57803-7.
Goats can provide meat, milk and skins for humans and are livestock with high economic benefits. However, despite their economic significance, the comprehensive analysis of goats' serum metabolic profile and its intricate alterations throughout their developmental journey remains conspicuously absent. To investigate the stage-specificity and dynamic change characteristics of metabolites during the growth and development of goats, this study compared the alterations in serum hormone levels and serum biochemical markers across different developmental stages of female goats (1, 60, 120 and 180 days old; n = 5). Additionally, a serum untargeted LC-MS metabolomics analysis was conducted. A total of 504 DAMs were identified with age. The results indicated that PE, PC, Lyso-PE, Lyso-PC and FAFHA may play important roles in lipid metabolism in goats after birth. Weighted gene co-expression network analysis (WGCNA) identified two metabolite modules (Turquoise and Yellow) and key metabolites within these modules that were significantly associated with phenotypic features. L-carnitine may be a metabolite related to muscle development in goats. The findings of this study demonstrate notable variations in serum metabolites across distinct developmental phases in goats. Lipids and organic acids play important roles in different developmental stages of goats.
山羊可以为人类提供肉、奶和皮，是经济效益很高的家畜。然而，尽管它们具有重要的经济意义，但山羊血清代谢谱的综合分析及其在整个发育过程中的复杂变化仍然明显缺失。为了研究山羊生长发育过程中代谢物的阶段特异性和动态变化特征，本研究比较了不同发育阶段（1、60、120 和 180 日龄；每组 5 只）雌性山羊血清激素水平和血清生化标志物的变化。此外，还进行了血清非靶向 LC-MS 代谢组学分析。共鉴定出 504 个 DAM，年龄相关。结果表明，PE、PC、Lyso-PE、Lyso-PC 和 FAFHA 可能在山羊出生后发挥重要作用。加权基因共表达网络分析（WGCNA）鉴定出两个代谢物模块（Turquoise 和 Yellow）和这些模块内与表型特征显著相关的关键代谢物。L-肉碱可能是山羊肌肉发育相关的代谢物。本研究结果表明，山羊在不同发育阶段的血清代谢物存在显著差异。脂质和有机酸在山羊的不同发育阶段发挥重要作用。
