Department of Surgical Sciences, Head and Neck Surgery, Section of Otolaryngology, Uppsala University Hospital, Uppsala, Sweden.
Department of Otolaryngology, Uppsala University Hospital, Uppsala, Sweden.
Ear Hear. 2020 Jan/Feb;41(1):173-181. doi: 10.1097/AUD.0000000000000738.
To three-dimensionally reconstruct Rosenthal's canal (RC) housing the human spiral ganglion (SG) using synchrotron radiation phase-contrast imaging (SR-PCI). Straight cochlear implant electrode arrays were inserted to better comprehend the electro-cochlear interface in cochlear implantation (CI).
SR-PCI was used to reconstruct the human cochlea with and without cadaveric CI. Twenty-eight cochleae were volume rendered, of which 12 underwent cadaveric CI with a straight electrode via the round window (RW). Data were input into the 3D Slicer software program and anatomical structures were modeled using a threshold paint tool.
The human RC and SG were reproduced three-dimensionally with artefact-free imaging of electrode arrays. The anatomy of the SG and its relationship to the sensory organ (Corti) and soft and bony structures were assessed.
SR-PCI and computer-based three-dimensional reconstructions demonstrated the relationships among implanted electrodes, angular insertion depths, and the SG for the first time in intact, unstained, and nondecalcified specimens. This information can be used to assess stimulation strategies and future electrode designs, as well as create place-frequency maps of the SG for optimal stimulation strategies of the human auditory nerve in CI.
利用同步辐射相衬成像（SR-PCI）对人类螺旋神经节（SG）所在的 Rosenthal 管（RC）进行三维重建。插入直线型耳蜗植入电极阵列，以更好地理解耳蜗植入（CI）中的电-耳蜗界面。
使用 SR-PCI 对有和没有尸体 CI 的人耳蜗进行重建。对 28 个耳蜗进行容积渲染，其中 12 个通过圆窗（RW）进行了尸体 CI，采用直线电极。将数据输入 3D Slicer 软件程序，并使用阈值涂漆工具对解剖结构进行建模。
成功地对 RC 和 SG 进行了三维重现，并且对电极阵列进行了无伪影成像。评估了 SG 的解剖结构及其与感觉器官（Corti）和软、硬组织结构的关系。
首次在完整、未染色和非脱钙标本中，通过 SR-PCI 和基于计算机的三维重建，展示了植入电极、角插入深度与 SG 之间的关系。这些信息可用于评估刺激策略和未来的电极设计，以及为 CI 中人类听神经的最佳刺激策略创建 SG 的放置-频率图。
